SAB4200740

Anti-Neurofilament 160 antibody, Mouse monoclonal

clone NN18, purified from hybridoma cell culture

• Synonym(s): Anti-160 kDa neurofilament protein, Anti-NF-M, Anti-Neurofilament 3, Anti-Neurofilament triplet M protein
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: NN18, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~160 kDa
• concentration: ~1.0 mg/mL
• technique(s): immunoblotting: 0.3-0.6 μg/mL using rat brain extract; immunofluorescence: 10-20 μg/mL using human bone marrow neuroblast SHSY5Y cell line; immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human brain sections
• isotype: IgG1
• UniProt accession no.: P07197
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Anti-Neurofilament 160 antibody, Mouse monoclonal, (mouse IgG1 isotype) is derived from the NN18 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mouse immunized with neurofilaments purified from pig spinal cord. Neurofilaments (NFs) belong to the intermediate filament (IF) family and are expressed mainly in cells or tissues of neuronal origin. Three major NFs subunits were discovered: light (NF-L, ~68 kDa), medium (NF-M, ~160 kDa) and heavy (NF-H, ~200 kDa), which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. Neurofilament 160, also known as NF-M or Neurofilament triplet M protein.

Immunogen

neurofilaments purified from pig spinal cord

Application

Anti-Neurofilament 160 antibody has been used in immunoblotting, Immunohistochemistry, Immunofluorescence

Biochem/physiol Actions

NFs can accumulate in large numbers within cell bodies and proximal axons of affected neurons in a variety of pathologies, including Charcot-Marie-Tooth disease (CMT), neurofilament inclusion disease (NFID), giant axonal neuropathy (GAN), diabetic neuropathy, spinal muscular atrophy (SMA) and spastic paraplegia. In addition, NFs accumulation were detected in Alzheimer′s (AD) and Parkinson′s disease (PD) patients. Elevated concentrations of Neurofilament 160 have been shown in cerebrospinal fluid (CSF) and serum samples from patients with brain injury.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable