SAE0094
SAvPhire™ Monomeric Streptavidin
recombinant, expressed in E. coli, High Affinity
Synonym(s):
mSA3
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.25
Recommended Products
recombinant
expressed in E. coli
form
lyophilized powder
shipped in
wet ice
storage temp.
−20°C
Biochem/physiol Actions
SAvPhire™ Monomeric Streptavidin is a variant of the biotin-binding streptavidin, which naturally exists as a tetramer. Many monomeric streptavidin proteins have rapid dissociation kinetics, which prevent their use in stable labeling applications. However, SAvphire™ Monomeric Streptavidin has been engineered to have a dissociation rate comparable to that of tetrameric streptavidin.
The size and monovalency of this product provide advantages over tetrameric streptavidin in applications such as functional cell surface labeling and atomic force spectroscopy. These advantages include:
This product can be used in traditional biotin-binding applications, including Western blotting, immunoprecipitation, immunohistochemistry, and flow cytometry.
- Minimizing distribution or dynamics in the probing of biotinylated targets
- Reducing risk of aggregation, precipitation, and cross-linking in pre-incubation steps with biotinylated molecules
- Easier access to desired target
This product can be used in traditional biotin-binding applications, including Western blotting, immunoprecipitation, immunohistochemistry, and flow cytometry.
Physical properties
Sequence contains N-terminal 6×-Histidine-tag and a C-terminal FLAG-tag
Reconstitution
Once reconstituted, the solution can be stored at 2-8 °C for at least 1 month. For long term storage, aliquot and store at -20 °C.
This product is lyophilized from PBS, pH 7.4. Reconstitute the vial contents to a 4 mg/mL solution by adding water as follows:
The reconstituted product can be further diluted in water or PBS as needed.
- 1 mg vial: add 250 μL of water for a 4 mg/mL solution
- 5 mg vial: add 1.25 mL of water for a 4 mg/mL solution
The reconstituted product can be further diluted in water or PBS as needed.
Legal Information
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
SAvPhire is a trademark of Sigma-Aldrich Co. LLC
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Functional expression of monomeric streptavidin and fusion proteins in Escherichia coli: applications in flow cytometry and ELISA.
Kroetsch, A. et al.
Applied Microbiology and Biotechnology, 102, 10079-10089 (2018)
Expression and purification of soluble monomeric streptavidin in Escherichia coli
Demonte D, et al.
Applied Microbiology and Biotechnology, 98(14), 6285-6295 (2014)
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