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Key Documents

SAE0180

Sigma-Aldrich

3CL, Mpro protease fluorogenic substrate peptide

AFC-Peptide substrate, ≥95% (HPLC), solid

Synonym(s):

3CL protease substrate

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.83

product name

3CL, Mpro protease fluorogenic substrate peptide, AFC-Peptide substrate, lyophilized

Quality Level

Assay

≥95% (HPLC)

form

solid

shipped in

ambient

storage temp.

−20°C

General description

This fluorogenic peptide is a substrate for the Main protease from SARS-Cov2, 3CL protease (Mpro). This substrate allows measurement of the activity of 3CL protease using a standard plate reader or spectrophotometer. Proteolytic cleavage by 3CL protease will release the fluorescent AFC group from the peptide (sequence TSAVLQ). After release, an increase in fluoresence is obtained (Excitation = 400 nm, Emission = 505 nm).

Peptide: Thr-Ser-Ala-Val-Leu-Gln-AFC

Lyophilized from 0.1% TFA in H2O

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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