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recombinant
expressed in baculovirus infected Sf9 cells
Assay
70-90% (SDS-PAGE)
form
frozen liquid
mol wt
~115 kDa
concentration
0.2 μg/μL
accession no.
BC065227
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... STAT5B(6777)
Application
Useful for Western blot.
Biochem/physiol Actions
Recombinant full-length human STAT5B was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. STAT5B or signal transducer and activator of transcription 5B is a member of the STAT family of transcription factors. STATs are phosphorylated by the receptor associated kinases and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5B is activated in response to a variety of cytokines (1). The JAK/STAT pathway mediates a key signal from the somatic gonad that regulates male germline sexual development (2). STAT5B is critical for maintenance of tolerance in vivo and it is proposed that STAT5B is probably activated by IL2R.
Reconstitution
Formulated in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
Storage and Stability
Store in aliquots at -70°C. Avoid freeze/thaw cycles.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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