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SRP5348

Sigma-Aldrich

GST protein, tag-free

recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

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About This Item

CAS Number:
UNSPSC Code:
12352202
NACRES:
NA.32

recombinant

expressed in E. coli

Assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

mol wt

~25 kDa

technique(s)

ligand binding assay: suitable

NCBI accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

Escherichia coli IAI39 ... gst> gst
gst(7152360)

General description

Glutathione S-transferase (GST) family of enzymes comprises a long list of cytosolic, mitochondrial and microsomal proteins that are used to create the so-called GST gene fusion system. These proteins are capable of multiple reactions with a multitude of substrates. They have a role in detoxication and toxification mechanisms. GST tag has the size of 220 amino acids, which is relatively large compared to other tags like the myc- or the FLAG-tag that are quite small. GST can be used to purify and detect proteins of interest. In a GST gene fusion system, an expression vector consists of the gene sequence encoding the protein of interest and GST sequence alongside. After induction, the fusion proteins can be purified using affinity chromatography via its high affinity for glutathione.

Application

GST protein, tag-free has been used in in vitro F-actin binding assay. It has also been used as a protein tag for TPX2 (targeting protein for Xklp2).

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

Preparation Note

After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Nanami Kikuchi et al.
ACS synthetic biology, 11(4), 1389-1396 (2022-04-05)
We present a cell-free assay for rapid screening of candidate inhibitors of protein binding, focusing on inhibition of the interaction between the SARS-CoV-2 Spike receptor binding domain (RBD) and human angiotensin-converting enzyme 2 (hACE2). The assay has two components: fluorescent
Italia Anna Asteriti et al.
Oncotarget, 8(19), 32117-32133 (2017-04-09)
Aurora kinases are a family of cell division regulators that govern the correct assembly of a bipolar mitotic spindle and the fidelity of chromosome segregation. Their overexpression is associated with genomic instability and aneuploidy, and is frequently observed in cancer.
Cdc42EP3/BORG2 and Septin Network Enables Mechano-transduction and the Emergence of Cancer-Associated Fibroblasts.
Calvo F
Cell Reports, 13(12), 2699-2714 (2015)
Identification of small molecule inhibitors of the Aurora-A/TPX2 complex
Asteriti IA, et al.
Testing, 8(12), 32117-32117 (2017)
Duong Thi Thuy Nguyen et al.
Cell death and differentiation, 24(6), 1063-1078 (2017-04-22)
Rapidity and specificity are characteristic features of proteolysis mediated by the ubiquitin-proteasome system. Therefore, the UPS is ideally suited for the remodeling of the embryonic stem cell proteome during the transition from pluripotent to differentiated states and its inverse, the

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