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T5912

Sigma-Aldrich

Tris Buffered Saline

10X solution

Synonym(s):

TBS

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About This Item

MDL number:
UNSPSC Code:
12161700
NACRES:
NA.25

product name

Tris Buffered Saline, 10 ×, solution

sterility

0.2 μm filtered

Quality Level

form

solution

concentration

10 ×

storage temp.

2-8°C

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Application

Tris Buffered Saline has been used as washing buffer for electrodes. It was used to wash microtiter plates for western blot experiments and screening methods.

Other Notes

Washing buffer for alkaline phosphatase or peroxidase conjugates in Western blotting. Working solution will contain 20 mM Tris, pH approx. 7.4, and 0.9% NaCl

Suitability

Suitable for use as a washing buffer for alkaline phosphatase or peroxidase conjugates in dot blot assay.

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Larry H Stanker et al.
Journal of immunological methods, 336(1), 1-8 (2008-05-03)
Botulinum neurotoxins (BoNT), produced by the anaerobic bacterium Clostridium botulinum, cause severe neuroparalytic disease and are considered the most toxic biological agents known. While botulism is rare in the U.S. it often is fatal if not treated quickly, and recovery
Rapid prototyping of multifunctional microfluidic cartridges for electrochemical biosensing platforms
Kin J
Sensors and Actuators B, Chemical, 202, 60-66 (2014)
Lucia Trisolini et al.
Biomolecules, 11(1) (2021-01-06)
ADP/ATP carriers (AACs) are mitochondrial transport proteins playing a strategic role in maintaining the respiratory chain activity, fueling the cell with ATP, and also regulating mitochondrial apoptosis. To understand if AACs might represent a new molecular target for cancer treatment
Edward Greenfield et al.
eLife, 3 (2014-12-11)
The Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by conducting replications of 50 papers in the field of cancer biology published between 2010 and 2012. This Registered Report describes the proposed replication plan
Jingtao Zhong et al.
International journal of molecular medicine, 41(5), 2893-2900 (2018-02-14)
Secretory clusterin (sCLU) is overexpressed in cancer and is associated with resistance to chemotherapy in several types of cancer, including hepatocellular carcinoma (HCC). Sorafenib (SOR), a multikinase inhibitor of Raf/mitogen‑activated protein kinase kinase/extracellular signal‑regulated kinase (ERK) signaling and the receptor

Articles

This solution is used as a substrate for detecting horseradish-peroxidase conjugates in Western blotting.

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