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V8379

Sigma-Aldrich

Human Vitronectin

from human plasma, powder, suitable for cell culture

Synonym(s):

Serum spreading factor

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About This Item

CAS Number:
83380-82-9
MDL number:
MFCD00132575
UNSPSC Code:
12352202
NACRES:
NA.75

product name

Vitronectin from human plasma, lyophilized powder, BioReagent, suitable for cell culture

biological source

human plasma

Quality Level

300

product line

BioReagent

form

lyophilized powder

mol wt

A subunit 75 kDa
B subunit 65 kDa

packaging

pkg of 50 μg

technique(s)

cell culture | mammalian: suitable

surface coverage

0.1 μg/cm2

solubility

water: soluble 0.050 mg/mL, clear, colorless

UniProt accession no.

P04004

shipped in

ambient

storage temp.

2-8°C

Gene Information

human ... VTN(7448)

Application

Vitronectin can be used with cells with integrin receptors that bind vitronectin, including platelets, endothelial cells, melanoma cells, and osteosarcoma. It is recommended as a cell culture substratum at 0.1 μg/cm2. Optimal conditions for attachment depend on cell line and application.

Biochem/physiol Actions

Vitronectin binds to glycosaminoglycans, is incorporated as an inhibitor to the membrane cytolytic attack complex of the complement system, has interaction with thrombin and antithrombin III during coagulation, and may play a role in the coagulation pathway.

Components

Vitronectin is a glycoprotein present in plasma and tissues, and is one of the major cell adhesion proteins, along with fibronectin, in plasma. The N-terminal residues of vitronectin are identical to somatomedin B and are followed by an R-G-D sequence that interacts with a specific cell-surface receptor. The central domain of vitronectin is enriched with hydrophobic residues. Closer to the C-terminus, it has an arginine rich region that allows for heparin binding activity.

Caution

The lyophilized product should be stored desiccated at 2-8°C. Under these conditions it is stable for at least 2 years. Aliquots should be stored at -20°C or -70°C, but repeated freezing and thawing should be avoided.

Preparation Note

This product is supplied as a cell culture tested lyophilized powder from buffered saline. It is recommended to use polypropylene vessels during handling, due to the strong affinity of vitronectin for hydrophilic surfaces. Reconstitution of this product can be done with 1 mL of water per vial. The stock solution should then be filter-sterilized using a 0.2μm membrane and aliquoted and frozen for long term storage.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Theodora Chatzisideri et al.
Journal of medicinal chemistry, 65(1), 271-284 (2021-12-31)
c(RGDyK)-based conjugates of gemcitabine (GEM) with the carbonate and carbamate linkages in the 6-OH group of GEM were synthesized for the targeted delivery of GEM to integrin αvβ3, overexpressing cancer cells to increase the stability as well as the tumor
Lorenzo Baldini et al.
Molecules (Basel, Switzerland), 27(4) (2022-02-26)
During tumor angiogenesis different growth factors, cytokines and other molecules interact closely with each other to facilitate tumor cell invasion and metastatic diffusion. The most intensively studied as molecular targets in anti-angiogenic therapies are vascular endothelial growth factor (VEGF) and
Francesca Posa et al.
Biomaterials, 267, 120484-120484 (2020-11-04)
Here we present the use of surface nanopatterning of covalently immobilized BMP-2 and integrin selective ligands to determine the specificity of their interactions in regulating cell adhesion and focal adhesion assembly. Gold nanoparticle arrays carrying single BMP-2 dimers are prepared
Lacey M Field et al.
Journal of phycology, 54(2), 299-303 (2017-12-19)
Vitronectin (Vn) is a glycoprotein that serves a wide variety of roles in multicellular organisms. It was first identified in multicellular animals but has also been isolated from land plants and some algae, where it appears to serve as an
The receptor for urokinase-plasminogen activator (uPAR) controls plasticity of cancer cell movement in mesenchymal and amoeboid migration style.
Margheri F, Luciani C, Taddei ML, et al.
Oncotarget, 5(6), 1538-1553 (2014)
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