Y1250
Yeast Nitrogen Base With Amino Acids
Non-selective yeast growth medium
Synonym(s):
ynb with amino acids
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About This Item
UNSPSC Code:
41106212
NACRES:
NA.85
Recommended Products
grade
for molecular biology
Quality Level
sterility
non-sterile
form
powder
technique(s)
microbiological culture: suitable
pH
5.3-5.5(0.67% solution)
application(s)
microbiology
storage temp.
room temp
suitability
nonselective for Candida spp.
nonselective for Pichia spp.
nonselective for Saccharomyces spp.
nonselective for Zygosaccharomyces spp.
General description
Yeast Nitrogen Base is a highly-referenced growth medium used for the cultivation of yeast. This nutrient-rich microbial broth contains amino acids, nitrogen, vitamins, trace elements and salts.
Application
Yeast Nitrogen Base With Amino Acids has been used:
Suitable for use in classifying yeasts based on carbon requirements. Also suitable for general growth of yeasts with the addition of a carbon source (usually glucose).Yeast nitrogen base with amino acids is a satisfactory nitrogen source for general growth media. Contains all essential nutrients required for the growth of yeasts except carbohydrate.
- in the ZYP-31 media preparation for E.coli
- in synthetic dextrose (SD) medium to evaluate the influence of nitrogen source
- to culture Candida utilis for the production of 13C-labeled yeast extract as internal standard
- in the preparation of imaging medium
Suitable for use in classifying yeasts based on carbon requirements. Also suitable for general growth of yeasts with the addition of a carbon source (usually glucose).Yeast nitrogen base with amino acids is a satisfactory nitrogen source for general growth media. Contains all essential nutrients required for the growth of yeasts except carbohydrate.
Components
Component, amount (μg/L unless indicated)
Nitrogen Sources:
Ammonium sulfate, 5.0 g/L
Amino Acids:
L-histidine, 10 mg/L
DL-methionine, 20 mg/L
DL-tryptophan, 20 mg/L
Vitamins:
Biotin, 2.0
Calcium pantothenate, 400
Folic acid, 2.0
Inositol, 2.0 mg/L
Nicotinic acid, 400
p-Aminobenzoic acid, 200
Pyridoxine HCl, 400
Riboflavin, 200
Thiamine HCL, 400
Trace Elements:
Boric acid
Copper sulfate
Potassium iodide
Ferric chloride
Magnesium sulfate
Sodium molybdate
Zinc sulfate
Salts:
Potassium phosphate monobasic, 1.0 g/L
Magnesium sulfate, 0.5 g/L
Sodium chloride, 0.1 g/L
Calcium chloride, 0.1 g/L
Nitrogen Sources:
Ammonium sulfate, 5.0 g/L
Amino Acids:
L-histidine, 10 mg/L
DL-methionine, 20 mg/L
DL-tryptophan, 20 mg/L
Vitamins:
Biotin, 2.0
Calcium pantothenate, 400
Folic acid, 2.0
Inositol, 2.0 mg/L
Nicotinic acid, 400
p-Aminobenzoic acid, 200
Pyridoxine HCl, 400
Riboflavin, 200
Thiamine HCL, 400
Trace Elements:
Boric acid
Copper sulfate
Potassium iodide
Ferric chloride
Magnesium sulfate
Sodium molybdate
Zinc sulfate
Salts:
Potassium phosphate monobasic, 1.0 g/L
Magnesium sulfate, 0.5 g/L
Sodium chloride, 0.1 g/L
Calcium chloride, 0.1 g/L
Reconstitution
Make a 10x concentrated stock solution by stirring to suspend 6.7g yeast nitrogen base powder and 5 g glucose in 100 mL water. Warm if necessary to aid solubilization. Filter sterilize and store at 2-8°C.
Dilute the 10x concentrated stock to a 1x working solution by adding 100mL concentrated stock to 900mL sterile water.
Dilute the 10x concentrated stock to a 1x working solution by adding 100mL concentrated stock to 900mL sterile water.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Protocols
Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.
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