ZRB1275
Anti-CD81 Antibody, clone 1C23 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
1C23, monoclonal
recombinant monoclonal
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt ~25.81 kDa
observed mol wt ~19 kDa
species reactivity
human
species reactivity (predicted by homology)
monkey
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
affinity binding assay: suitable
flow cytometry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG
UniProt accession no.
greener alternative category
, Aligned
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 1C23 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects CD81. It targets an epitope within 23 amino acids from the first extracellular domain.
Immunogen
KLH-conjugated linear peptide corresponding to 23 amino acids from the first extracellular domain of human CD81.
Application
Quality Control Testing
Evaluated by Western Blotting in Human Testis tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CD81 in Human Testis tissue lysate.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CD81 in human tonsil tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound CD81 peptides with a KD of 9.8 x 10-8 in an affinity binding assay.
Flow Cytometry Analysis: 1 μg from a representative lot detected CD81 in one million MOLT4 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in Human Testis tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CD81 in Human Testis tissue lysate.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CD81 in human tonsil tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound CD81 peptides with a KD of 9.8 x 10-8 in an affinity binding assay.
Flow Cytometry Analysis: 1 μg from a representative lot detected CD81 in one million MOLT4 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-CD81, clone 1C23 ZooMAb®, Cat. No. ZRB1275, is a recombinant Rabbit monoclonal antibody that specifically detects CD81 and is tested for use in Affinity Binding Assay, Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting.
Target description
CD81 antigen (UniProt: P60033; also known as 26 kDa cell surface protein TAPA-1, Target of the antiproliferative antibody 1, Tetraspanin-28, Tspan-28, CD81) is encoded by the CD81 (also known as TAPA1, TSPAN28) gene (Gene ID: 975) in human. TAPA-1 is a nonglycosylated, homodimeric, multi-pass membrane protein of the tetraspanin (TM4SF) family. It is expressed in a wide range of cells, including hematolymphoid, neuroectodermal, and mesenchymal tumor cell lines and is reported to play a role in the regulation of lymphoma cell growth. It contains four transmembrane domains, two extracellular loops, and short amino and carboxyl termini that are intracellular. TAPA-1 is shown to affect cell adhesion, morphology, activation, proliferation, and differentiation of B, T, and other cells. On B cells it is a part of a complex involving CD21, CD19, and Leu13 and this complex is reported to reduce the threshold for B cell activation via the B cell receptor by bridging antigen specific recognition and CD21-mediated complement recognition. On T cells, it is shown to associate with CD4 and CD8 and provides a costimulatory signal with CD3. It is also reported to act as a receptor for hepatitis C virus (HCV) in hepatocytes. Mutations in CD81 gene cause common variable immunodeficiency 6 (CVID6) that is characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections, and an inability to mount an antibody response to antigen. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Levy S., et al. (1998). Annu. Rev. Immunol.16; 89-109; Oren, R., et al. (1990). Mol. Cell Biol. 10(8); 4007-15).
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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