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Key Documents

G005797

HPLC Analysis of Aspirin and Salicylic Acid on Ascentis® Express RP-Amide

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis Express RP-Amide, 10 cm x 4.6 mm I.D., 2.7 μm particles (53929-U)
mobile phase: [A] water with 0.1% TFA; [B] acetonitrile with 0.1% TFA; (65:35, A:B)
flow rate: 1 mL/min
pressure: 2200 psi (152 bar)
column temp.: 30 °C
detector: UV, 250 nm
injection: 5 μL
sample: 50 μg/mL in 75:25, water:acetonitrile

suitability

application for HPLC

application(s)

pharmaceutical (small molecule)

Analysis Note

Salicylic acid is the primary metabolite of aspirin, and is likely responsible for its antiinflammatory properties via suppression of cyclooxygenase genes; it also is thought to suppress colonic carcinogenesis. Ascentis Express RP-Amide provides good retention and resolution at low pH by a combination of hydrophobic and H-bonding intereactions.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Acetylsalicylic acid, analytical standard
    Salicylic acid, BioXtra, ≥99.0%

analytical column

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standard

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