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Merck

AB5491

Anti-Calcium Channel Cav3.1 Antibody

Chemicon®, from rabbit

Sinónimos:

T-type Alpha 1G

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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
WB
Citations:
1
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biological source

rabbit

Quality Segment

conjugate

unconjugated

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat

manufacturer/tradename

Chemicon®

technique(s)

western blot: suitable

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

Immunogen

Highly purified peptide corresponding to residues 1-22 of rat Cav3.1 (Accession O54898).

Application

Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters

Neuroinflammation & Pain
This Anti-Calcium Channel Cav3.1 Antibody is validated for use in WB for the detection of Calcium Channel Cav3.1.
Western blotting: 1:200 using ECL on rat brain membranes. See suggested protocol.

Dilutions should be made using a carrier protein such as BSA (1-3%).

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Recognizes Cav3.1. The epitope specific for Cav3.1 is not present in any other known proteins.

SPECIES REACTIVITIES:

The immunogen sequence is conserved in human (20/22). Reactivity with other species has not yet been tested.

Physical form

Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA, 5% sucrose and 0.05% sodium azide. Reconstitute with 200 μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).

Preparation Note

Maintain lyophilized material at -20°C for up to 6 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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hcodes

Hazard Classifications

Aquatic Chronic 3

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3



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