E9884

Ethylenediaminetetraacetic acid

ACS reagent, 99.4-100.6%, powder

• Synonyma: (Ethylenedinitrilo)tetraacetic acid, EDTA, Edathamil, Ethylenedinitrilotetraacetic acid
• Lineární vzorec: (HO₂CCH₂)₂NCH₂CH₂N(CH₂CO₂H)₂
• Číslo CAS: 60-00-4
• Molekulová hmotnost: 292.24
• NACRES: NA.77
• PubChem Substance ID: 24894705
• eCl@ss: 39030907
• UNSPSC Code: 12352106
• EC Number: 200-449-4
• MDL number: MFCD00003541
• Beilstein/REAXYS Number: 1716295

Vlastnosti

• grade: ACS reagent
• Quality Segment: 200
• agency: suitable for SM 4500 - NH3
• assay: 99.4-100.6%
• form: powder
• reaction suitability: reagent type: chelator
• impurities: ≤0.005% insoluble matter, ≤0.1% Nitrilotriacetic acid ([HOCOCH₂]₃N)
• ign. residue: ≤0.2%
• pH: 2.5 (23 °C, 10 g/L)
• mp: 250 °C (dec.) (lit.)
• solubility: water: soluble 0.4 g/L at 20 °C
• cation traces: Ca: ≤0.001%, Fe: ≤0.005%, Mg: ≤5 ppm, heavy metals: ≤0.001% (byICP-OES)
• SMILES string: OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
• InChI: 1S/C10H16N2O8/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20)
• InChI key: KCXVZYZYPLLWCC-UHFFFAOYSA-N

Popis

General description

Ethylenediaminetetraacetic acid (EDTA) is a hydrophilic metal chelating agent that transforms metal ions into inactive, cyclic metal complexes. Hence, it has industrial application for resolving metal contamination.

Application

Ethylenediaminetetraacetic acid has been used:

• as a component of labeling buffer, which is used in the washing step during cell fractionation{155)
• for investigating the effect of papain enzyme on wound debridement and other skin issues
• for harvesting rat arterial smooth muscle cell after its pre-treatment for its use in flow cytometry analysis

Biochem/physiol Actions

Ethylenediaminetetraacetic acid (EDTA) is not easily administered by the gastrointestinal tract. Intravenous administration of sodium EDTA results in hypocalcemic tetany. It is generally useful in plasma or molecular diagnostics and acts by chelating calcium. EDTA serves as an anticoagulant for haematological cell counting and cell morphological analysis.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Bezpečnostní informace

• signalword: Warning
• Skladovací třída: 11 - Combustible Solids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves
• target_organs: Respiratory Tract
• pictograms: GHS08,GHS07
• hcodes: H319,H332,H373
• pcodes: P260 - P264 - P271 - P304 + P340 + P312 - P305 + P351 + P338 - P314
• Hazard Classifications: Acute Tox. 4 Inhalation - Eye Irrit. 2 - STOT RE 2 Inhalation