77332

Peroxidase from horseradish

lyophilized, powder, ~150 U/mg

• Synonyma: (2R,3S,6S,7R,8R)-3-(3-formamido-2-hydroxybenzamido)-8-hexyl-2,6-dimethyl-4,9-dioxo-1,5-dioxonan-7-yl 3-methylbutanoate, Antimycin A, Horseradish peroxidase
• Číslo CAS: 9003-99-0
• NACRES: NA.54
• UNSPSC Code: 12352204
• EC Number: 232-668-6
• MDL number: MFCD00071339
• Číslo EC: 1.11.1.7 (BRENDA, IUBMB)
• eCl@ss: 32160410
• Specific activity: ~150 U/mg
• Biological source: horseradish

Vlastnosti

• biological source: horseradish
• Quality Segment: 100
• form: powder
• quality: lyophilized
• specific activity: ~150 U/mg
• technique(s): protein detection: suitable
• solubility: 50 mM potassium phosphate buffer, pH 6.0: 5 mg/mL (0.1% BSA)
• shipped in: wet ice
• storage temp.: 2-8°C
• SMILES string: [O+H2]O[O-]
• InChI: 1S/H2O3/c1-3-2/h1-2H
• InChI key: JSPLKZUTYZBBKA-UHFFFAOYSA-N

Popis

General description

Horseradish peroxidase (HRP) is a vital and a widely studied heme-containing enzyme, isolated from a hardy perennial herb, horse radish. It catalyzes the oxidation of various organic and inorganic compounds by using hydrogen peroxide as substrates. HRP is involved in indole-3-acetic acid metabolism, cross-linking of biological polymers and lignification. This enzyme in combination with indole-3-acetic acid or its derivatives acts as a potential therapeutic for targeted cancer therapies. Commercially HRP is used as a constituent of clinical diagnostic kits and is used in immunoassays.

Application

Horseradish peroxidase is used widely within biochemistry as a detection reagent in a variety of applications including ELISA, immunoblotting, and immunohistochemistry.

Peroxidase from horseradish has been used:

• for isolation of N-Linked glycans
• as a constituent of D-amino acid oxidase (DAAO) buffer
• to decompose hydrogen peroxide (H2O2) into water to demonstrate the performance of the portable oxidative stress sensor (POSS)

Other Notes

1 U corresponds to the amount of enzyme which oxidizes 1 μmol ABTS (Cat. No. 11557) per minute at pH 6.0 and 25°C

Use of chemically modified peroxidase in benzene; Cross-linking of proteins; Enzyme catalyzed polymerization and precipitation of aromatic compounds from wastewater.

Bezpečnostní informace

• Piktogramy: GHS08
• Výstražné slovo: Danger
• Kódy nebezpečí: H334
• Bezpečnostní pokyny: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Skladovací třída: 13 - Non Combustible Solids
• Co se děje?: WGK 1
• PPE (osobní ochranné prostředky): Eyeshields, Gloves, type N95 (US)