M0275

Minimum Essential Medium Eagle

With Earle′s salts, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture, 10x

• Synonyma: EMEM, MEM
• UNSPSC Code: 12352207
• NACRES: NA.71

Vlastnosti

• Název produktu: Minimum Essential Medium Eagle, 10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
• Quality Segment: 400
• sterility: sterile-filtered
• form: liquid
• concentration: 10 ×
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• components: phenol red: yes; HEPES: no; Earle’s salts (5% CO₂): yes; L-glutamine: no; sodium pyruvate: no; NaHCO₃: no
• shipped in: ambient
• storage temp.: 2-8°C

Popis

Application

Generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera. The L-glutamine and sodium bicarbonate are added to the medium after dilution to 1x and pH adjustment.

Minimum Essential Medium Eagle has been used for the formulation of collagen methacrylamide (hydrogel). It has also been used to maintain articular cartilage of bovine knee joints, which was later used for MRI (magnetic resonance imaging) study and histological analysis.

Preparation Note

Supplement with 0.292 g/L L-glutamine, 2.2 g/L sodium bicarbonate at 1×.

Bezpečnostní informace

• Skladovací třída: 12 - Non Combustible Liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable