SHC016H
MISSION® pLKO.1-puro Non-Target shRNA Control Transduction Particles, High Titer
Targets no known genes from any species
Synonyma:
MISSION®, MISSION® Control Transduction Particles, negative control, negative shRNA control, non-target control, non-target shRNA, non-target shRNA control, shRNA control
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O této položce
NACRES:
NA.51
UNSPSC Code:
41106609
Concentration:
≥1x109 VP/ml (via p24 assay)
Technický servis
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product line
MISSION®
concentration
≥1x109 VP/ml (via p24 assay)
shipped in
dry ice
storage temp.
−70°C
General description
The MISSION pLKO.1-puro Non-Target shRNA Control Transduction Particles, High Titer contain an shRNA insert that does not target any known genes from any species, making it useful as a negative control in experiments using the MISSION shRNA library clones. This allows one to examine the effect of transduction of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids.
High titer virus is useful when performing in vivo research.
High titer virus is useful when performing in vivo research.
When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.
To see more application data, protocols, vector maps visit sigma.com/shrna.
Application
To see more application data, protocols, vector maps visit sigma.com/shrna.
Legal Information
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
Skladovací třída
12 - Non Combustible Liquids
Co se děje?
WGK 3
Bod vzplanutí (°F)
Not applicable
Bod vzplanutí (°C)
Not applicable
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