D5905

3,3′-Diaminobenzidine tetrahydrochloride

peroxidase substrate, chromogenic, tablet

• Synonym(s): 3,3′,4,4′-Biphenyltetramine tetrahydrochloride, 3,3′,4,4′-Tetraaminobiphenyl tetrahydrochloride, DAB
• Linear Formula: (NH₂)₂C₆H₃C₆H₃(NH₂)₂ · 4HCl
• Molecular Weight: 360.11
• NACRES: NA.83
• PubChem Substance ID: 24893981
• UNSPSC Code: 12352204
• EC Number: 231-018-9
• MDL number: MFCD00012969
• Beilstein/REAXYS Number: 1212988

Properties

• Product Name: 3,3′-Diaminobenzidine tetrahydrochloride, tablet, 10 mg substrate per tablet
• Quality Segment: 200
• form: tablet
• technique(s): protein detection: suitable
• solubility: water: 1 tablet/15 mL (may contain small insoluble particles)
• storage temp.: −20°C
• SMILES string: Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2
• InChI: 1S/C12H14N4.4ClH/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;/h1-6H,13-16H2;4*1H
• InChI key: KJDSORYAHBAGPP-UHFFFAOYSA-N

Description

Application

Reagent for spectrophotometric determination of selenium.

3,3′-Diaminobenzidine tetrahydrochloride has been used for immunohistochemistry.

Biochem/physiol Actions

Peroxidase substrate suitable for use in immunoblotting and immunohistological staining procedures. This substrate produces an insoluble end product that is brown in color and can be observed visually.

3,3′-Diaminobenzidine tetrahydrochloride is a chromogenic substrate, which is commonly used in immunoperoxidase tests.

Preparation Note

Dissolve 1 tablet in 15 ml of Tris-buffered saline, pH 7.6. Add 12 μl of fresh 30% hydrogen peroxide. DAB and hydrogen peroxide concentration may be adjusted to suit individual applications.

Other Notes

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Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Danger
• Hazard Codes: H302,H319,H341,H350
• Precautionary Statements: P202 - P264 - P280 - P301 + P312 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• PPE (personal protective equipment): Eyeshields, Gloves, type N95 (US)