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Millipore

GranuCult® BAT agar

pkg of 500 g, according to IFU 12, for Alicyclobacillus spp.

Synonym(s):

Alicyclobacillus selective agar, Bacillus acidoterrestris agar

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About This Item

UNSPSC Code:
41106213
NACRES:
NA.74

Agency

according to IFU 12

Quality Level

200

sterility

non-sterile

form

granular

packaging

pkg of 500 g

manufacturer/tradename

GranuCult® prime

storage condition

protect from light (both UV and Sun light)

technique(s)

microbiological culture: suitable

application(s)

food and beverages
microbiology

storage temp.

15-25°C

suitability

Alicyclobacillus spp. (spp.)

General description

GranuCult® BAT agar supports the growth of Alicyclobacillus spp. These are aerobic, Gram-positive spore-forming bacteria, with optimum growth at low pH and increased temperatures. Alicyclobacilli are spoilage organisms, especially affecting the quality of fruit juices.
GranuCult® BAT agar contains glucose as a carbon and energy source. Yeast extract is the source of vitamins, particularly the B-group. Potassium dihydrogen phosphate acts as a buffering system. The low pH and the high incubation temperature inhibit the contaminating flora. This medium contains many trace elements, which supply the specific requirements of the Alicyclobacillus spp. agar is the solidifying agent however, the gel strength is weak due to low pH.

Application

GranuCult® BAT agar is suitable for the enumeration, detection, and identification of Alicyclobacillus spp. from fruit juices, other beverages, and their ingredients, environmental samples, including process water and other materials in the area of food production and food handling.

Analysis Note

Appearance (clearness): slightly turbid to turbid
Appearance (color): yellowish
pH-value (25 °C): 3.9 - 4.1
Growth (Alicyclobacillus acidocaldarius DSMZ 446): +
Growth (Alicyclobacillus acidoterrestris DSMZ 2498): +
Growth (Alicyclobacillus cycloheptanicus DSMZ 4006): +
Growth (Alicyclobacillus hesperidium DSMZ 12766): +
Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): -
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): -
Incubation: A.acidocaldarius 72 hrs.; 60 °C aerobic; other strains 72 hrs.; 45 °C.
Reference for Staph.aureus und E.coli blood agar (Incubation: 24 hrs.; 35 °C).

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

CAS No.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Types of Media in Microbiology

The different types of culture media, that are used to grow microorganisms in the laboratory for quality control, are classified by several criteria, such as consistency, composition, or selectivity.

Revised IFU Method No. 12 for Alicyclobacillus spp.

New microbiological standards for fruit juices focus on detecting Alicyclobacillus spp. using BAT broth and agar.

Related Content

Alicyclobacillus Spoilage in Juices: Testing Methods

Alicyclobacillus are acidophilic, strictly aerobic, and spore-forming spoilage bacteria that can cause increased turbidity, color changes and unpleasant sensory properties in juices. Testing is performed according to IFU method No 12 (2019).

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