AB6017

Anti-F-actin-capping protein subunit beta Antibody

from rabbit

• Synonym(s): capping protein (actin filament) muscle Z-line, beta, F-actin capping protein beta subunit
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: mouse, rat, human
• species reactivity (predicted by homology): canine (based on 100% sequence homology), primate (based on 100% sequence homology), bovine (based on 100% sequence homology)
• technique(s): immunocytochemistry: suitable; western blot: suitable
• NCBI accession no.: NP_004921
• UniProt accession no.: P47756
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: bovine ... Capzb(338052) ; dog ... Capzb(478209) ; human ... CAPZB(832) ; mouse ... Capzb(12345) ; rat ... Capzb(298584)

Description

General description

F-actin-capping-protein subunit beta (CapZ beta) belongs to the F-actin capping protein family, which are characteristic heterodimers, consisting of an α subunit (31-36 kDa) and a β subunit (28-32 kDa), that cap the barbed end of actin filaments within all eukaryotes. Their ability to bind the actin filaments is in a manner independent of Ca2++ and requires the C-terminal end of both subunits for optimal binding. F-actin capping protein subunit beta is contained within the Z-discs of striated muscle, where it functions to inhibit the polymerization and depolymerization of actin. Within vertebrates, there are three isoforms for each of F-actin capping protein subunit beta’s subunits. While there is almost nothing known regarding the β3 subunit, the β1 and β2 isoforms each have distinct roles. The isoform β2 is predominantly expressed in non-muscle cells and is found in intercalated discs and the cell periphery, but is not observed at Z-discs. Isoform β1 is more greatly expressed in striated muscle and is localized at the Z-discs. It also contains the COOH-terminal extension necessary for capping the actin.

Specificity

The antibody recognizes F-actin-capping protein subunit beta at the C-terminus of isoform 2.

Immunogen

Epitope: C-terminus of Isoform 2

KLH-conjugated linear peptide corresponding to human F-actin-capping protein subunit beta of the C-terminus of isoform 2.

Application

Detect F-actin-capping protein subunit beta using this Anti-F-actin-capping protein subunit beta Antibody validated for use in WB & IC.

Immunocytochemistry Analysis: 1:500 dilution of the antibody has been shown to detect F-actin-capping protein subunit beta in NIH/3T3 and A431 cells.

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected F-actin-capping protein subunit beta in 10 µg of HeLa cell lysate.

Target description

~ 34 kDa observed MW. There are 3 isoforms produced by alternative splicing: Isoform 1 at 31 kDa, Isoform 2 at 31 kDa, and Isoform 3 (sequence not available).

Physical form

Format: Purified

Protein A purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl, with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable