LIF3010
Leukemia Inhibitory Factor Protein, Recombinant rat
Recombinant rat Leukemia Inhibitory Factor (LIF) protein is biologically active and suitable for cell culture applications.
Synonym(s):
LIF
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About This Item
UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.75
Recommended Products
biological source
rat
Quality Level
Assay
>85% (HPLC and SDS-PAGE)
form
liquid
specific activity
≥1 x 10(e8) U/mg
manufacturer/tradename
Chemicon®
concentration
10 μg/mL
technique(s)
cell culture | stem cell: suitable
impurities
<0.1 ng/μg Endotoxin (of LIF)
input
sample type: mouse embryonic stem cell(s)
sample type mesenchymal stem cell(s)
sample type hematopoietic stem cell(s)
sample type neural stem cell(s)
sample type induced pluripotent stem cell(s)
UniProt accession no.
shipped in
dry ice
General description
Leukemia Inhibitory Factor (LIF) is a lymphoid factor which promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo.
Product Source: rtLIF is expressed in E. coli as a fusion protein with GST using the pGEX expression system, cleaved from GST moiety with thrombin and purified by ion-exchange chromatography.
Rat LIF (rtLIF) is a 22.1 kDa protein containing 202 amino acid residues that exhibits 91% amino acid sequence identity with murine LIF(Takahama etal 1998).Analysis of the secondary structure of rat and murine LIF indicates a difference in the two dimensional structure of both proteins. Studies have shown that rtLIF is more effective in maintaining the undifferentiated phenotype of rat ES cells than similar concentrations of murine LIF (Takahama, et al 1998).
Application
RECOMMENDED QC PROTOCOL
M1 Bioassay
1.The M1 bioassay is performed using in vitro semi-solid agar cultures, which contain approximately 100 cells in 1 mL volumes of DME containing 20 % FCS in 0.3% agar.
2.Add 100 μL of sample or rtLIF (10(e4) units/mL in 5% FCS in isotonic saline) in two-fold serial dilutions in duplicate to 35 mm petri dishes.
3.Add 100 μL of 5% FCS in isotonic saline to two control slides.
4.Incubate at 37°C in fully humidified atmosphere of 10% CO2 in air for 7 days.
5.Score the number of colonies that show differentiation (note: 50 units is defined as the amount of activity which results in 50% of the colonies being differentiated).
Visit www.esgro-lif.com for additional information
M1 Bioassay
1.The M1 bioassay is performed using in vitro semi-solid agar cultures, which contain approximately 100 cells in 1 mL volumes of DME containing 20 % FCS in 0.3% agar.
2.Add 100 μL of sample or rtLIF (10(e4) units/mL in 5% FCS in isotonic saline) in two-fold serial dilutions in duplicate to 35 mm petri dishes.
3.Add 100 μL of 5% FCS in isotonic saline to two control slides.
4.Incubate at 37°C in fully humidified atmosphere of 10% CO2 in air for 7 days.
5.Score the number of colonies that show differentiation (note: 50 units is defined as the amount of activity which results in 50% of the colonies being differentiated).
Visit www.esgro-lif.com for additional information
Unit Definition
Specific Activity: 50 units is defined as the amount of rat LIF required to induce differentiation in 50% of the M1 colonies in 1 mL agar cultures.
Physical form
Liquid in 50mM sodium phosphate/1mM DTT / 10% glycerol / 250mM NaCl, pH 7.4 and 0.02% Tween 20. No preservativies added.
Storage and Stability
Rat LIF is shipped on dry ice. Maintain at -20°C until expiration date. Further dilutions should be made into buffer or medium to which protein (e.g., 1% BSA) or Tween 20 has been added and stored at -20°C. Freeze thawing is not recommended.
Analysis Note
Specific Activity: The activity of rat LIF is determined by the ability to induce differentiation of M1 myeloid leukemic cells.The minimum detectable concentration of rat LIF in this assay is 0.5 ng/mL
Tested negative in both aseptic and mycoplasma tests.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Leukaemia inhibitory factor alters expression of genes involved in rat cardiomyocyte energy metabolism.
Florholmen, G, et al.
Acta Physiologica Scandinavica, 180, 133-142 (2004)
Przemyslaw Blyszczuk et al.
Methods in molecular biology (Clifton, N.J.), 330, 373-385 (2006-07-19)
Embryonic stem (ES) cells represent a potential source of transplantable cells for the treatment of diabetes because of their almost unlimited proliferation capacity and the potential to differentiate into insulin-producing cells. The differentiation conditions significantly affect the development of pancreatic
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