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MAB1637

Sigma-Aldrich

Anti-Tubulin Antibody, beta III isoform, CT, clone TU-20 (Similar to TUJ1)

ascites fluid, clone TU-20 (Similar to TUJ1), Chemicon®

Synonym(s):

Tubulin beta-III, tubulin, beta 3, tubulin, beta 4

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

ascites fluid

antibody product type

primary antibodies

clone

TU-20 (Similar to TUJ1), monoclonal

species reactivity

rat, mouse, avian, human, bovine, monkey, pig, sheep

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_006086.2

UniProt accession no.

Q13509

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... TUBB3(10381)

General description

Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins which makes up microtubules. Each has a molecular weight of approximately 55 kDa. Microtubules are assembled from dimers of α- and β-tubulin. These subunits are slightly acidic with an isoelectric point between 5.2 and 5.8. Tubulin was long thought to be specific to eukaryotes. Recently, however, the prokaryotic cell division protein FtsZ was shown to be evolutionarily related to tubulin.

Specificity

Expected to cross-react with Avian, Porcine (Pig), Rat, Bovine, Sheep, Human, Monkey. Reactivity with other species has not been confirmed.
The antibody reacts with the C-terminus of the neuron specific beta III isoform of tubulin. Does not identify beta tubulin found in glial cells.

Immunogen

A synthetic peptide corresponding to amino acids 443-450 (ESEAQGPK) of human class III beta-tubulin conjugated to KLH.
Epitope: C-terminus

Application

Anti-Tubulin Antibody, beta III isoform, C-terminus, clone TU-20 (Similar to TUJ1) is an antibody against Tubulin for use in ELISA, IC, IH, IH(P), IP & WB with more than 55 product citations.
Immunohistochemistry:
On formalin fixed frozen or paraffin embedded tissue sections: 1:20 to 1:100 dilution from a previous lot. High temperature citrate buffer antigen retrieval is recommended with paraffin embedded tissue sections.

Immunoprecipitation:
A previous lot of this antibody was used on Immunoprecipitation.

Immunocytochemistry:
1:100 to 1:500 dilution from a previous lot was used on fixed cells.

Immunoblotting:
1:1000. Antibody will detect a single band at 50 kDa corresponding to class III beta-tubulin.

ELISA:
A previous lot of this antibody was used on ELISA.

Optimal working dilution must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers

Quality

Routinely evaluated by Western Blot on Mouse Brain lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected tubulin (βIII) on 10 μg of Mouse Brain lysates.

Target description

55 kDa

Linkage

Replaces: 04-1049

Physical form

Mouse monoclonal IgG1 ascites in buffer containing PBS containing 1% BSA and 15 mM sodium azide.
Unpurified

Storage and Stability

Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Positive control: Brain tissue or neurons of any type.
Negative control: Non neural tissue or Astrocytes.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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