17226

Universal Beer Agar

suitable for microbiology, NutriSelect^® Plus

• Synonym(s): UB Agar, UBA medium
• UNSPSC Code: 41171605
• NACRES: NA.74

Properties

• sterility: non-sterile
• Quality Level: 200
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: agar, 12 g/L ; dextrose, 16.1 g/L ; dipotassium phosphate, 0.31 g/L ; ferrous sulfate, 0.006 g/L ; magnesiumsulfate, 0.12 g/L ; manganese sulfate, 0.006 g/L ; monopotassium phosphate, 0.31 g/L ; peptonized milk, 15 g/L ; sodium chloride, 0.006 g/L ; tomato juice, 12.2 g/L ; yeast extract, 6.1 g/L
• manufacturer/tradename: NutriSelect^® Plus
• packaging: pkg of -500 g
• technique(s): microbiological culture: suitable
• final pH: 6.3±0.2 (25 °C)
• application(s): bioburden testing; food and beverages; microbiology
• suitability: Acinetobacter spp.; Pediococcus spp.; selective for Candida spp.; selective for Escherichia coli; selective for Lactobacillus spp.; selective for Pichia spp.; selective for Proteus spp.; selective for Saccharomyces spp.; selective for Zygosaccharomyces spp.; selective for coliforms

Description

General description

Universal Beer Agar is a base medium supplemented with beer. Its characteristics are closer to natural environmental conditions found in a typical brewery. Thus, it supports the growth of more lactic acid bacteria and yields larger colonies in a shorter time than other brewer’s media. The addition of beer gives the medium some selectivity to the organisms adapted to existing conditions in the brewery. Hop and alcohol inhibit the growth of many other microorganisms, which are not adapted to the beer matrix.

Application

Universal Beer Agar is suitable for culturing spoilage microorganisms of significance in the brewing industry (bacteria like Lactobacillus, Pediococcus, Acetobacter, and yeasts).

Preparation Note

Suspend 62.16 g in 750 ml of distilled water. Heat to boiling to dissolve the medium completely. Add 250 ml beer, without degassing, to the hot medium and mix gently. Dispense as desired and sterilize by autoclaving at 121°C for 10 minutes. If required, add 1 ug/ml (Cat. No. 01810) of Cycloheximide to sterile medium prior to dispensing.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable