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39441

Millipore

Nitrate Reagent B

suitable for microbiology

Synonym(s):

Sulfanilic acid solution, 4-Aminobenzenesulfonic acid

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About This Item

CAS Number:
121-57-3
MDL number:
MFCD00007886
UNSPSC Code:
41171621
PubChem Substance ID:
329755871
NACRES:
NA.85

shelf life

limited shelf life, expiry date on the label

Quality Level

100

composition

acetic acid (30%), 1000 mL
sulfanilic acid, 8.0 g

technique(s)

microbe id | metabolite detection: suitable

application(s)

agriculture
clinical testing
environmental
food and beverages
pharmaceutical
microbiology

suitability

Enterobacter spp.
Escherichia coli
Neisseria spp.
anaerobic bacteria
coliforms

SMILES string

Nc1ccc(cc1)S(O)(=O)=O

InChI

1S/C6H7NO3S/c7-5-1-3-6(4-2-5)11(8,9)10/h1-4H,7H2,(H,8,9,10)

InChI key

HVBSAKJJOYLTQU-UHFFFAOYSA-N

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Application

α-Naphthylamine and sulfanilic acid are used for detection of nitrate reduction by bacteria. Organisms containing nitrate reductase reduce nitrate to nitrite, which forms a diazonium salt with sulfanilic acid and reacts with α-naphthylamine to form a red azo dye.

Pictograms

Corrosion
GHS05

Signal Word

Danger

Hazard Statements

H314

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCL6942
BCCJ0564
BCCD0792
BCBV9795
BCBM6557V

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Han Ming Gan et al.
Microbiology (Reading, England), 158(Pt 8), 1933-1941 (2012-05-23)
The gene coding for the oxygenase component, sadA, of 4-aminobenzenesulfonate (4-ABS) 3,4-dioxygenase in Hydrogenophaga sp. PBC was previously identified via transposon mutagenesis. Expression of wild-type sadA in trans restored the ability of the sadA mutant to grow on 4-ABS. The
Han Ming Gan et al.
Chemosphere, 82(4), 507-513 (2010-11-26)
A co-culture consisting of Hydrogenophaga sp. PBC and Ralstonia sp. PBA, isolated from textile wastewater treatment plant could tolerate up to 100 mM 4-aminobenzenesulfonate (4-ABS) and utilize it as sole carbon, nitrogen and sulfur source under aerobic condition. The biodegradation
Kwan Kim et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 100, 10-14 (2012-02-14)
We estimated the apparent size of the 'hot site' for surface-enhanced Raman scattering (SERS) located within the gaps between Pt nanoparticles and a flat Ag substrate. Initially, no Raman peaks were detected for 4-aminobenzenethiol (4-ABT) on a flat Ag substrate.
Han Ming Gan et al.
FEMS microbiology letters, 318(2), 108-114 (2011-02-18)
Genes involved in the 4-aminobenzenesulfonate (4-ABS) degradation pathway of Hydrogenophaga sp. PBC were identified using transposon mutagenesis. The screening of 10,000 mutants for incomplete 4-ABS biotransformation identified four mutants with single transposon insertion. Genes with insertions that impaired the ability
Brian D Piorek et al.
Proceedings of the National Academy of Sciences of the United States of America, 104(48), 18898-18901 (2007-11-21)
We present a microfluidic technique for sensitive, real-time, optimized detection of airborne water-soluble molecules by surface-enhanced Raman spectroscopy (SERS). The method is based on a free-surface fluidic device in which a pressure-driven liquid microchannel flow is constrained by surface tension.
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