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66721

Sigma-Aldrich

Methylene Blue zinc chloride double salt

for microscopy (Bact., Bot., Hist.), ~1 mol/mol water

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About This Item

Empirical Formula (Hill Notation):
C16H18ClN3S · 0.5ZnCl2 · xH2O
CAS Number:
26283-09-0
Molecular Weight:
388.00 (anhydrous basis)
Beilstein:
3839953
MDL number:
MFCD00150006
UNSPSC Code:
12352106
PubChem Substance ID:
329760311
NACRES:
NA.25

grade

for microscopy (Bact., Bot., Hist.)

Quality Level

100

impurities

~1 mol/mol water

SMILES string

O.[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C

InChI

1S/C16H18N3S.ClH.H2O/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;;/h5-10H,1-4H3;1H;1H2/q+1;;/p-1

InChI key

WQVSELLRAGBDLX-UHFFFAOYSA-M

Application

Methylene blue is a heterocyclic aromatic chemical compound used as a cationic dye. It is used as a redox indication in analytical chemistry. Methylene blue is a component of a number of staining protocols such as Wright′s stain and Jenner′s stain for microscopic evaluation.

Pictograms

Skull and crossbones
GHS06

Signal Word

Danger

Hazard Statements

H301,H319

Hazard Classifications

Acute Tox. 3 Oral - Eye Irrit. 2

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
404519/1

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Kristine Krafts et al.
Parasitology research, 109(3), 521-529 (2011-06-11)
Methylene blue was synthesized by Caro in 1876 at BASF, a chemical company. Six years later, Koch employed methylene blue when he discovered the tubercle bacillus. In 1880, Ehrlich described what he termed "neutral" dyes: mixtures of acidic and basic
K P Krafts et al.
Biotechnic & histochemistry : official publication of the Biological Stain Commission, 86(1), 7-35 (2011-01-18)
The components of the blood stain, eosin and methylene blue, were introduced by Baeyer and Caro, respectively. Methylene blue was used primarily for detecting Mycobacterium tuberculosis until Ehrlich in 1880 mixed methylene blue with acid fuchsin to produce what he
Mohammad A Al-Ghouti et al.
Scientific reports, 10(1), 15928-15928 (2020-09-29)
In the current study, the mechanistic understanding of the adsorption isotherm and thermodynamic aspects of cationic methylene blue (MB) dye adsorption onto cellulosic olive stones biomass from wastewater were investigated. The batch adsorption of MB onto the olive stones (black
A Van Deun et al.
The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease, 14(12), 1571-1575 (2010-12-15)
Damien Foundation Bangladesh tuberculosis (TB) control projects. To compare blue ink, potassium permanganate and methylene blue background staining for transmitted light-emitting diode (LED) TB fluorescence microscopy (FM). Auramine smears made in triplicate from Ziehl-Neelsen (ZN) acid-fast bacilli (AFB) positive or

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