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P4544

Sigma-Aldrich

Phenazine ethosulfate

≥95% purity, powder

Synonym(s):

N-Ethyldibenzopyrazine ethyl sulfate salt

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About This Item

Linear Formula:
C16H18N2SO4
CAS Number:
Molecular Weight:
334.39
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

product name

Phenazine ethosulfate, ≥95%

Quality Level

Assay

≥95%

form

powder

solubility

ethanol: 50 mg/mL, clear

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

CCOS([O-])(=O)=O.CC[n+]1c2ccccc2nc3ccccc13

InChI

1S/C14H13N2.C2H6O4S/c1-2-16-13-9-5-3-7-11(13)15-12-8-4-6-10-14(12)16;1-2-6-7(3,4)5/h3-10H,2H2,1H3;2H2,1H3,(H,3,4,5)/q+1;/p-1

InChI key

VDJKJPMLWJWQIH-UHFFFAOYSA-M

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General description

Phenazine ethosulfate is a cationic dye and a chemical electron acceptor. It is used in dye-linked enzyme assays. At a high pH, the dye produces free radicals, which may be the actual electron acceptors.

Application

Phenazine ethosulfate has been used:
  • in the spectrophotometric assessment of Plasmodium falciparum lactate dehydrogenase activity
  • to study the use of the dye during culture of embryos on fetal and postnatal calf development
  • to test and validate a fluorescence approach to quantify lipid content of individual bovine oocytes and blastocysts

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Cross-validation of techniques for measuring lipid content of
bovine oocytes and blastocysts
M. Barcelo
Theriogenology (2011)
Emanuele Zonaro et al.
Microbial cell factories, 16(1), 215-215 (2017-12-01)
Bacteria have developed different mechanisms for the transformation of metalloid oxyanions to non-toxic chemical forms. A number of bacterial isolates so far obtained in axenic culture has shown the ability to bioreduce selenite and tellurite to the elemental state in
Antiplasmodial properties of some Malaysian medicinal
plants
Tropical Biomedicine (2007)
Phenazine ethosulfate as a preferred electron acceptor to phenazine methosulfate in dye-linked enzyme assays
R. Ghosh
Analytical Biochemistry, 99(1) (1979)
High-Throughput Screening in Drug Discovery (2006)

Protocols

Objective: To standardize a procedure for the recycling micro-assay of β-NAD and β-NADH

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