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RDD035

Sigma-Aldrich

HEPES sodium salt

≥99.5% (titration), free-flowing, Redi-Dri™

Synonym(s):

HEPES Sodium, HEPES hemisodium salt, Sodium HEPES, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt

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About This Item

Empirical Formula (Hill Notation):
C8H17N2NaO4S
CAS Number:
75277-39-3
Molecular Weight:
260.29
MDL number:
MFCD00036463
UNSPSC Code:
12161700
NACRES:
NA.25

product line

Redi-Dri™

Quality Level

100

Assay

≥99.5% (titration)

quality

free-flowing

useful pH range

6.8-8.2

pKa (25 °C)

7.5

application(s)

diagnostic assay manufacturing

SMILES string

[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O

InChI

1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1

InChI key

RDZTWEVXRGYCFV-UHFFFAOYSA-M

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General description

HEPES sodium salt is essential to prepare HEPES buffer that is commonly used for cell culture and protein applications. It is useful for maintaining homeostasis when working with cells outside of the incubator by balancing CO2. HEPES buffering capacity exceeds most buffers, with a pKa of 7.5, making it optimal for maintaining pH in biological systems. In solution, it is non-toxic, zwitterion with a physiological buffering range of pH 6.8 - 8.2. It has a low metal binding capacity and is safe to use in research for metal-binding proteins.

Application

HEPES sodium salt can be used:
  • as a protein precipitation buffer
  • in cell culture media
  • as a binding buffer

Features and Benefits

  • Redi-Dri™ eliminates clumping for hygroscopic chemicals
  • powder remains free-flowing and easy to use
  • Safer to handle and weigh out for standard use in lab
  • Eliminates clumps, maintains product moisture, quality, and characteristics

Physical form

Lyophilized, spray dried, or crystallized material.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SLCF3906

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Nishant Mehta et al.
Cell reports, 28(10), 2509-2516 (2019-09-05)
V-domain immunoglobulin (Ig) suppressor of T cell activation (VISTA) is an immune checkpoint protein that inhibits the T cell response against cancer. Similar to PD-1 and CTLA-4, a blockade of VISTA promotes tumor clearance by the immune system. Here, we report a
Åsa Birna Birgisdottir et al.
Autophagy, 15(8), 1333-1355 (2019-02-16)
Autophagosome formation depends on a carefully orchestrated interplay between membrane-associated protein complexes. Initiation of macroautophagy/autophagy is mediated by the ULK1 (unc-51 like autophagy activating kinase 1) protein kinase complex and the autophagy-specific class III phosphatidylinositol 3-kinase complex I (PtdIns3K-C1). The
Peng Yang
Macromolecular bioscience, 12(8), 1053-1059 (2012-06-19)
When lysozyme is dissolved in a neutral HEPES buffer solution (pH = 7.4) with 0.001-0.050 M TCEP added, a fast phase transition process occurs and the resulting novel fiber-like hierarchical supramolecular assemblies made by primary spherical-particle aggregation can function as a "superglue"
Rakesh K Pathak et al.
Analytical chemistry, 84(11), 5117-5123 (2012-05-04)
An in situ prepared Zn(2+) complex of triazole linked imino-thiophenyl conjugate of calix[4]arene, [ZnL], was demonstrated to be highly fluorescent in HEPES buffer solution. [ZnL] has been used as a chemo-sensing ensemble for the recognition of phosphates in general and
Junbo Li et al.
Dalton transactions (Cambridge, England : 2003), 41(13), 3623-3626 (2012-02-24)
We developed a novel method for Cu(2+) detection based on the ion promoted oxidation reaction. Chemodosimeter L (weak fluorescence) can be oxidized into 3-benzothiazoly-7-N,N-diethylaminocoumarin (strong green fluorescence, coumarin 6) by Cu(2+) with high selectivity and sensitivity in HEPES (10 mM
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