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150-05F

Sigma-Aldrich

Human Skeletal Muscle Cells: HSkMC, fetal

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About This Item

UNSPSC Code:
41106514
NACRES:
NA.81

biological source

human skeletal muscle (normal limb)

Quality Level

100

packaging

pkg of 500,000 cells

manufacturer/tradename

Cell Applications, Inc

growth mode

Adherent

karyotype

2n = 46

morphology

Skeletal muscle

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

muscular dystrophy

shipped in

dry ice

storage temp.

−196°C

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Human Skeletal Muscle Cells (HSkMC) are isolated from the skeletal muscle of limbs from adult or fetal donors. Human Skeletal Muscle Cells can undergo differentiation to exhibit actin and myosin myofilaments. HSkMC provide a useful model system to study many aspects of muscular function and disease. Skeletal Muscle Cells play an instrumental role in the glucose metabolism and diabetes, therefore select HSkMC lots undergo an additional set of characterization to demonstrate AMPK signaling and responsiveness to insulin stimulation.

Cell Line Origin

Muscle (Limb)

Application

lipid and glucose metabolism, exercise, insulin action, cell signaling

Components

Basal Medium containing 10% FBS & 10% DMSO

Preparation Note

  • 3rd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
  • Can be cultured at least 15 doublings

Subculture Routine

Please refer to the HSkMC Culture Protocol.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Protocols

Human Skeletal Muscle Cells (HSkMC) Culture Protocol

Technical information for working with human skeletal muscle cells including thawing, subculturing and cryopreservation

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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