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Key Documents

A1047

Sigma-Aldrich

Anti-Mouse IgG (γ-chain specific)−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Goat Anti-Mouse IgG (γ-chain specific)−AP

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

recombinant

expressed in goat

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse

technique(s)

direct ELISA: 1:7,000-1:21,000
western blot: suitable

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

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General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.
Goat anti-mouse IgG (gamma-chain specific)-alkaline phosphatase antibody associates with mouse IgG (gamma-chain specific).

Immunogen

purified mouse IgG

Application

Antibodies to Trypanosoma cruzi were detected in the sera of infected mice by ELISA with an alkaline phosphatase conjugated goat anti-mouse IgG chain specific. Plates were coated with T. cruzi antigen and blocked with 1% BSA/PBS prior to incubation with conjugated antibody.
For detection of IgG antibodies in mouse sera and saliva following S. gordonii infection, ELISA assays were performed using anti-mouse IgG alkaline phosphatase antibody at 1:20000. This antibody did not detect IgA antibodies.
Goat anti-mouse IgG (gamma-chain specific)-alkaline phosphatase antibodies can be used for western blot assays,.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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