C7855
Anti-Cdh1 antibody,Mouse monoclonal
clone DCS-266, purified from hybridoma cell culture
Synonym(s):
Anti-CDC20C, Anti-CDH1, Anti-FZR, Anti-FZR2, Anti-HCDH, Anti-HCDH1
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About This Item
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biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
DCS-266, monoclonal
form
buffered aqueous solution
mol wt
antigen 50 kDa
species reactivity
human
concentration
~2 mg/mL
technique(s)
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 2-4 μg/mL using a HeLa cell nuclear extract
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CDH1(999)
General description
Cdh1 homophilic major cell adhesion molecule in epithelial cells belongs to cadherin superfamily.
Monoclonal Anti-Cdh1 (mouse IgG1 isotype) is derived from the DCS-266 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a recombinant human cadherin-1 (Cdh1).
Specificity
Monoclonal Anti-Cdh1 reacts specifically with human Cdh1.
Immunogen
recombinant human Cdh1.
Application
Monoclonal Anti-Cdh1 antibody was used for immunoblotting of lysates in a study of Identification of Novel Human Cdt1-binding Proteins. It is suitable for western blotting with 2-4 μg/mL by using a HeLa cell nuclear extract. It is also suitable for immunoprecipitation and microarray.
Biochem/physiol Actions
Monoclonal Anti-Cdh1 reacts specifically with human Cdh1. In eukaryotes, regulation of cell cycle progression depends on the expression of cyclins proteins. Degradation of mitotic cyclins follows ubiquitin-dependent pathways. The anaphase-promoting complex/cyclosome (APC/C) plays a vital role in progression. Initially at anaphase the APC/C requires the activator protein CDC20 to target securin and cyclin B1 for proteasome-dependent degradation, but later it require Cdh1 to maintain its active state till the next S phase. Another cyclin A/cdk2 mediated phosphorylation of Cdh1 may also prevents the activatory assembly of Cdh1 with APC, thus creating an environment permissive for accumulation of APC targets. It mediates Ca2+ dependent homophilic interactions, as the major adhesion receptor in adherens junctions.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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The tumor-suppressor function of E-cadherin.
H Semb et al.
American journal of human genetics, 63(6), 1588-1593 (1998-12-05)
Reinhard Sigl et al.
Journal of cell science, 122(Pt 22), 4208-4217 (2009-10-29)
The anaphase-promoting complex/cyclosome (APC/C) is essential for progression through mitosis. At anaphase onset, the APC/C requires the activator protein CDC20 to target securin and cyclin B1 for proteasome-dependent degradation, but then depends on the CDC20-related protein FZR1 (also known as
Suyang Zhang et al.
Nature, 533(7602), 260-264 (2016-04-28)
In eukaryotes, the anaphase-promoting complex (APC/C, also known as the cyclosome) regulates the ubiquitin-dependent proteolysis of specific cell-cycle proteins to coordinate chromosome segregation in mitosis and entry into the G1 phase. The catalytic activity of the APC/C and its ability
Xiaojing Zhang et al.
Oncology letters, 17(3), 2639-2646 (2019-03-12)
Overexpressed in lung cancer 1 (OLC1) is a potential oncogene overexpressed in human lung cancer and in other types of malignant tumor. The elevated expression of OLC1 contributes to tumor genesis and progression. However, the mechanisms regulating the expression of
Nozomi Sugimoto et al.
Molecular biology of the cell, 19(3), 1007-1021 (2007-12-29)
In mammalian cells, Cdt1 activity is strictly controlled by multiple independent mechanisms, implying that it is central to the regulation of DNA replication during the cell cycle. In fact, unscheduled Cdt1 hyperfunction results in rereplication and/or chromosomal damage. Thus, it
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