C8696

Cathepsin D from human liver

lyophilized powder, ≥250 units/mg protein (E1%/280)

• CAS Number: 9025-26-7
• Enzyme Commission number: 3.4.23.5 (BRENDA, IUBMB)
• MDL number: MFCD00130749
• UNSPSC Code: 12352204
• NACRES: NA.32

Properties

• form: lyophilized powder
• Quality Level: 200
• specific activity: ≥250 units/mg protein (E1%/280)
• mol wt: ~45 kDa
• color: white
• UniProt accession no.: P07339
• storage temp.: −20°C
• Gene Information: human ... CTSD(1509)

Description

General description

Cathepsin D is an aspartic protease, which is located in lysosomes. It is involved in protein catabolism and maintains hormone and antigen processing. Cathepsin D is implicated in neoplasia and neurodegenerative changes. It regulates lysosomal proteolysis and endogenous fibrinolysis.

Application

Cathepsin D from human liver has been used:

• in β-secretase activity assay
• for enzymatic degradation of amyloid β 1-42
• in microinjection of human foreskin fibroblasts

Biochem/physiol Actions

Cathepsin D is an endosomal-lysosomal aspartic protease implicated in breast cancer metastasis and Alzheimer′s disease. Lysosomal release of cathepsin D has been found to precede cytochrome c release and loss of membrane potential in apoptotic human foreskin fibroblasts. Cathepsin D levels in PC12 cells increase 12 to 24 hours after apoptosis is induced.

Other Notes

Contains α, β and γ isoenzymes as observed by isoelectric-focusing.

Unit Definition

One unit will produce an increase in A₂₈₀ of 1.0 in 30 min at pH 3.3 at 37 °C measured as TCA-soluble products using acid-denatured hemoglobin as substrate (1 cm light path).

Physical form

Powder containing sodium phosphate buffer salt.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable