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Key Documents

COO/COA

94A1

Name: 94A1
Brand: SIGMA

85062503, Application: Fusion partner

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About This Item

UNSPSC Code:

41106514

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P3X63Ag8U.1

BW5147.G.1.4.OUA/R.1

53B3

HF2x653

P3X63Ag8.653 Cell Line from mouse

P3X63Ag8

NS0 Cell Line from murine myeloma

45.6.TG1.7

WHA1823125

Whatman^® glass microfiber filters, Grade GF/D

P3U1

biological source

Mouse x Bovine Heterohybridoma

ImmunoDon

Jersey calf lymph node

Myeloma

94A1 (mouse x bovine heteromyeloma)

Reactivity

Application: Fusion partner

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Mouse 53B3 x Bovine Jersey calf lymph node cells

Cell Line Description

The antibody is directed against sheep red blood cells. It does not react with bovine red cells. This haemocytic antibody is probably directed against the Forssman hapten. This HAT sensitive line can be used as a fusion partner for the production of heterohybridomas.

Immunogen

Sheep red blood cells

Application

Fusion partner

The isotype of the monoclonal antibody produced by this cell line is bovine IgG1.

Culture Medium

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

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