D2625

Dansyl chloride

BioReagent, suitable for amino acid labeling, powder and chunks, ≥99% (HPLC)

• Synonym(s): 5-(Dimethylamino)naphthalene-1-sulfonyl chloride, DNSCl
• Empirical Formula (Hill Notation): C₁₂H₁₂ClNO₂S
• CAS Number: 605-65-2
• Molecular Weight: 269.75
• Beilstein: 2217205
• EC Number: 210-092-6
• MDL number: MFCD00003985
• UNSPSC Code: 12352101
• PubChem Substance ID: 24893700
• NACRES: NA.26

Properties

• product line: BioReagent
• Assay: ≥99% (HPLC)
• form: powder and chunks
• color: yellow to yellow-orange
• mp: 72-74 °C (lit.)
• solubility: acetone: 20 mg/mL
• suitability: suitable for amino acid labeling
• storage temp.: 2-8°C
• SMILES string: CN(C)c1cccc2c(cccc12)S(Cl)(=O)=O
• InChI: 1S/C12H12ClNO2S/c1-14(2)11-7-3-6-10-9(11)5-4-8-12(10)17(13,15)16/h3-8H,1-2H3
• InChI key: XPDXVDYUQZHFPV-UHFFFAOYSA-N

Description

Application

Dansyl chloride has been used:

• as a fluorogenic reagent for fluorescent N-terminal derivatization of amino acids and peptides and detection by reverse phase high performance liquid chromatography (HPLC)
• in the derivatization of plasma samples
• in the quantification of immobilized alkaline phosphatase

A fluorogenic reagent for fluorescent N-terminal derivatization of amino acids and peptides and detection by reverse phase HPLC.

Biochem/physiol Actions

Dansyl chloride is a sulfonyl chloride compound. It is used to end tag amino groups of peptides and proteins. Dansyl chloride is used for derivatization of free amino acids and amines in neural samples and biological fluids. It is used to study fluorescent conjugates of albumin, protein conformational changes studies and active site characterization.

Safety Information

• Pictograms: GHS05
• Signal Word: Danger
• Hazard Statements: H290,H314
• Precautionary Statements: P234 - P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B
• Storage Class Code: 8A - Combustible corrosive hazardous materials
• WGK: WGK 3
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves