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Key Documents

G7041

Sigma-Aldrich

Fish Gelatin

from cold water fish skin, powder, suitable for cell culture and generation of scaffolds.

Synonym(s):

Gelatine, Teleostean gelatin

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.75

product name

Gelatin from cold water fish skin, solid

biological source

fish skin

product line

BioReagent

form

solid

mol wt

60 kDa

packaging

pkg of 100 g
pkg of 500 g

technique(s)

cell culture | mammalian: suitable

surface coverage

100—200 μg/cm2

impurities

≤2% Residue on ignition (Ash)
≤20 ppm Heavy metals

solubility

acetic acid: soluble
glycerol: soluble
water: soluble

shipped in

ambient

storage temp.

room temp

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General description

Gelatin derived from cold water fish skin has low gelling and melting points. This gelatin will not gel at 10 degree celsius, hence they do not have bloom strength.

Application

Gelatin from cold water fish skin has been used in the preparation of water phase. It has also been used to maintain growth in static culture and in flavopiridol treatment and transcript labeling.
Used as a blocking agent in immunochemistry.

Biochem/physiol Actions

Gelatin from fish skin can be used in the preparation of various gels based on their gelling characteristics. It can also be used as an additive in surimi processing to enhance the functional and mechanical properties of gel.

Storage Class Code

11 - Combustible Solids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Using fish gelatin and pectin to make a low-fat spread.
Cheng L H, et al.
Food Hydrocolloids, 22(8), 1637-1640 (2008)
Marine Proteins and Peptides: Biological Activities and Applications (2013)
Encapsulation and Controlled Release Technologies in Food Systems (2016)
Maximising the Value of Marine By-Products (2006)
Samia Rourou et al.
Applied microbiology and biotechnology, 85(1), 53-63 (2009-06-13)
Vero cells growth and rabies production in IPT-AF medium, a property animal-component-free medium are described in this work. Kinetics of cell growth and rabies virus (strain LP 2061) production were first conducted in spinner flasks. Over eight independent experiments, Vero

Articles

The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.

Protocols

This gelatin coating protocol for cell culture applications offers information regarding types of gelatin, surface coverage concentration, and tips for optimization.

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