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GE17-0584-10

Sephacryl® S-200 HR

Cytiva 17-0584-10, pack of 150 mL

Synonym(s):

Sephacryl S-200

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About This Item

UNSPSC Code:
13111000
NACRES:
NA.56

packaging

pack of 150 mL

manufacturer/tradename

Cytiva 17-0584-10

matrix

allyl dextran and N, N’-methylenebisacrylamide

particle size

25-75 μm

avg. part. size

50 μm

cleaning in place

2-13

working range

3-11

suitability

suitable for bioprocess medium

General description

Sephacryl™ High Resolution size exclusion chromatography resins allow fast and reproducible purification of proteins, polysaccharides, and other macromolecules by size exclusion chromatography at laboratory and industrial scale.

Sephacryl™ High Resolution chromatography resins is a highly versatile gel filtration resins that offers a wide range of fractionation capabilities. The matrix of Sephacryl™ High Resolution resins is a cross-linked copolymer of allyl dextran and N,N’-methylene bisacrylamide. This crosslinking gives good rigidity and chemical stability. The narrow particle size distribution, together with steep selectivity curves, results in good preparative characteristics with maintained high resolution. The hydrophilic nature of the resins minimizes nonspecific adsorption and maximizes recovery. The excellent resolution and flow characteristics, long-term physical and chemical stability, and ease of handling make Sephacryl™ HR the resin of choice for routine purification.

Sephacryl™ S-100 High Resolution is designed for separating peptides and small proteins (fractionation range globular proteins 1 × 10³–1 × 10⁵). Sephacryl™ S-200 High Resolution are for purifying antibodies, serum proteins and mid-size proteins (fractionation range globular proteins 5 × 10³–2.5 × 10⁵). Sephacryl™ S-300 High Resolution are for purifying antibodies, serum proteins and mid-size proteins (fractionation range globular proteins 1 × 10⁴–1.5 × 10⁶). Sephacryl™ S-400 High Resolution to separate polysaccharides, macromolecules with extended structures and even small particles such as plasmids. (fractionation range globular proteins 2 × 10⁴–8 × 10⁶). Sephacryl™ S-500 High Resolution to separate polysaccharides, macromolecules with extended structures and even small particles such as plasmids (fractionation range Dextrans 4 × 10⁴–2 × 10⁷)

Features and Benefits

  • Cost-efficient gel filtration resins providing high resolution with fast and easy operation.
  • Five different fractionation ranges available in Sephacryl™ HR family allows for reproducible separations over a wide molecular weight range.
  • Sephacryl™ S-200 High Resolution resin is designed for purifying antibodies, serum proteins and mid-size proteins (fractionation range globular proteins 5 × 10³ – 2.5 × 10⁵).
  • BioProcess™ resin supported for industrial applications and well-established in approved processes.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sephacryl is a registered trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Storage Class Code

3 - Flammable liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

Sephacryl column separates using Size Exclusion Chromatography media, prepared by covalently cross-linking allyl dextran.

Column Packing and Preparation for Size Exclusion Chromatography

Related Content

Separation options for Size Exclusion Chromatography with Sephacryl from Cytiva.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service