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GE28-9893-32

Sigma-Aldrich

HiLoad® 26/600 Superdex® 30 pg

Cytiva 28-9893-32, column L × I.D. 60 cm × 26 mm, 34 μm avg. part. size

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

material

bed support (polyamide)
glass column
glass fiber (polypropylene reinforced)

mol wt

<10000 Da (globular proteins)

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 28-9893-32

parameter

<13 mL sample volume
<2.6 mL/min flow rate
0.3 Mpa (42 psi)

bed volume

320 mL

column L × I.D.

60 cm × 26 mm

matrix

agarose-dextran composite
 dextran-agarose particle platform
polymeric

matrix active group

dextran-agarose phase
dextran-agarose phase

avg. part. size

34 μm

cleaning

1-14

working range

3-12

separation technique

size exclusion (SEC)

General description

HiLoad 26/600 Superdex 30 prep grade are prepacked XK columns designed for high-resolution preparative gel filtration chromatography separating peptides.

Suitability

All commonly used buffers, 1 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile
for high-resolution preparative gel filtration chromatography separating peptides

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Legal Information

HiLoad is a registered trademark of Cytiva
Superdex is a registered trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Setting Pressure Limits for Size Exclusion Chromatography

Understanding pressure drop in chromatography systems is crucial for optimal functionality across different parts of the system.

Protocols

Performing a Separation with Superdex

Superdex from Cytiva are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.

Sample Preparation for Size Exclusion Chromatography

Ensure chromatographic sample clarity to avoid column clogging, reduce washing needs, and prolong column life for purification.

Related Content

Separation Options with Superdex®

Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.

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