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H2126

Sigma-Aldrich

Hyaluronidase from sheep testes

Type II, lyophilized powder, ≥300 units/mg solid

Synonym(s):

Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase

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About This Item

CAS Number:
37326-33-3
Enzyme Commission number:
3.2.1.35 (BRENDA, IUBMB)
EC Number:
253-464-3
MDL number:
MFCD00131351
UNSPSC Code:
12352204
NACRES:
NA.54

conjugate

conjugate (Glucosaminoglycan)

type

Type II

form

lyophilized powder

specific activity

≥300 units/mg solid

mol wt

55 kDa

storage temp.

−20°C

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General description

Hyaluronidase is a hyaluronic acid-metabolizing enzyme. It is found in the venoms of snake, honeybee and hymenoptera. Hyaluronidase degrades hyaluronic acid (HA), which enhances membrane permeability, lowers viscosity and increases tissue permeability. It enhances the diffusion of local anesthetics, lowers the size of myocardial infarction, improves the effects of anticancer drugs and prevents perineal trauma during spontaneous delivery.

Application

Hyaluronidase from sheep testes has been used:
  • in flow cytometry
  • in the isolation of putative spermatogonial stem cells (SSCs)
  • to study the effects of magnetic stimulation in magnetic hydrogel properties
  • in immunohistochemistry to visualize collagen type II distribution
  • to expose group-III auditory neurons of Muller′s organ for patch-clamp recordings
  • for the degeneration of hydrogels

Biochem/physiol Actions

These enzymes randomly cleave β-N-acetylhexosamine-(1−4)-glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Unit Definition

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

Physical form

Lyophilized powder containing lactose

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Yield stress determines bioprintability of hydrogels based on gelatin-methacryloyl and gellan gum for cartilage bioprinting
Mouser VHM, et al.
Biofabrication, 8(3), 035003-035003 (2016)
Robert B Struijk et al.
PloS one, 15(3), e0230253-e0230253 (2020-03-17)
Autologous transplantation of spermatogonial stem cells is a promising new avenue to restore fertility in infertile recipients. Expansion of the initial spermatogonial stem cell pool through cell culturing is a necessary step to obtain enough cells for effective repopulation of
3D bioprinting of methacrylated hyaluronic acid (MeHA) hydrogel with intrinsic osteogenicity
Poldervaart MT, et al.
PLoS ONE, 12(6), e0177628-e0177628 (2017)
Progenitor cells in auricular cartilage demonstrate cartilage-forming capacity in 3D hydrogel culture
Otto IA, et al.
European Cells & Materials, 35, 132-150 (2018)
The role of the mechanotransduction ion channel candidate Nanchung-Inactive in auditory transduction in an insect ear.
Warren B and Matheson T
The Journal of Neuroscience, 2310-2317 (2018)
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Glycosaminoglycans and Proteoglycans

Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.

Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

Protocols

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

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