L1882

Lectin from Triticum vulgaris (wheat)

agarose conjugate, saline suspension

• Synonym(s): WGA, Wheat germ agglutinin
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• conjugate: agarose conjugate
• Quality Level: 200
• form: saline suspension
• extent of labeling: 5-10 mg per mL
• matrix: cross-linked 4% beaded agarose
• matrix activation: cyanogen bromide
• capacity: ≥0.1 mg/mL binding capacity (ovomucoid)
• storage temp.: 2-8°C

Description

General description

Lectins are carbohydrate-binding proteins, found in fungi, plants and animals. The secondary structure of a lectin is rich in β-strands and possesses a carbohydrate binding sites on the surface.

Application

Used for the purification of glycoproteins containing (D-N-acetylglucosamine)₂ or N-acetylneuraminic acid.

Biochem/physiol Actions

Lectins are known to be useful in glycoconjugate characterizing, imaging and targeting. They specifically interact with oligosaccharides, glycoproteins and glycolipids. Hence, lectins are used in microarray assays, enabling efficient glycome profiling. In plants and fungi, lectins defend against pathogens or feeders. They are also involved in host recognition and tissue adhesion.

WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.

Physical form

Suspension in 1.0 M NaCl containing 15 mM sodium azide

Preparation Note

Conjugates are prepared from affinity purified lectin.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves