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L6274

Sigma-Aldrich

Laminin from human placenta

liquid, BioReagent, suitable for cell culture

Synonym(s):

Cell culture grade laminin, Human Laminin

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About This Item

CAS Number:
114956-81-9
MDL number:
MFCD00081739
UNSPSC Code:
12352202
NACRES:
NA.77

biological source

human placenta

Quality Level

300

product line

BioReagent

form

liquid

concentration

0.5 mg/mL in Tris-buffered saline

technique(s)

cell culture | mammalian: suitable

surface coverage

1‑2 μg/cm2

UniProt accession no.

P07942

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... LAMB1(3912)

Application

Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. It is recommended for use as a cell culture substratum at 1-2 μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.

Biochem/physiol Actions

Laminin proteins are integral components of structural scaffolding in animal tissues. They associate with type IV collagen via entactin and perlecan and bind to cell membranes through integrin receptors, dystroglycan glycoprotein complexes and Lutheran blood group glycoproteins. Laminin has active domains for collagen binding, cell adhesion, heparin binding, and neurite outgrowth fragment.

Components

Laminin is an extracellular matrix multidomain trimeric glycoprotein, and is the main non-collagenous component of basal lamina that supports adhesion, proliferation and differentiation. Laminin is composed of both A, B1 and B2 chains, which are connected by many disulfide bonds. This laminin product is purified from human placenta using pepsin treatment and immunoaffinity chromatography. SDS-PAGE results in two major bands at 50 kDa and 130-160 kDa.

Caution

It is recommended to store this product at -70°C, where it will remain stable for two years.

Preparation Note

This product is supplied at a concentration of 0.5 mg/mL in 50 mM Tris-HCl, with 150 mM NaCl. It is 0.2 μm filtered. Thaw this solution slowly before use at 2-8°C to avoid gel formation. For use as a coating, dilute in a sterile balanced salt solution, coat culture surface with a minimal volume and incubate at 37°C for two hours. Wash 3 times with PBS before plating cells. Laminin coatings can be stored for one month at 2-8°C.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Satu Marja Myllymäki et al.
PloS one, 6(5), e19453-e19453 (2011-05-17)
Formation of apical compartments underlies the morphogenesis of most epithelial organs during development. The extracellular matrix (ECM), particularly the basement membrane (BM), plays an important role in orienting the apico-basal polarity and thereby the positioning of apical lumens. Integrins have
Terhi P Teräväinen et al.
PloS one, 8(8), e71485-e71485 (2013-08-27)
The properties of epithelial cells within tissues are regulated by their immediate microenvironment, which consists of neighboring cells and the extracellular matrix (ECM). Integrin heterodimers orchestrate dynamic assembly and disassembly of cell-ECM connections and thereby convey biochemical and mechanical information
Danielle K Lewis et al.
Aging cell, 7(6), 836-849 (2008-09-10)
Astrocytes comprise a large proportion of the central nervous system support cells and play a critical role in neural injury and repair. The present study examined the impact of ovarian aging using an ex vivo model system, where astrocytes were
Chiyi Xiong et al.
Scientific reports, 10(1), 520-520 (2020-01-18)
The tyrosine kinase receptor EphB4 is frequently overexpressed in ovarian and other solid tumors and is involved in interactions between tumor cells and the tumor microenvironment, contributing to metastasis. Trans-interaction between EphB4 and its membrane-bound ligand ephrin B2 (EFNB2) mediates
Sanne L N Brouns et al.
Scientific reports, 10(1), 11910-11910 (2020-07-19)
In haemostasis and thrombosis, platelet, coagulation and anticoagulation pathways act together to produce fibrin-containing thrombi. We developed a microspot-based technique, in which we assessed platelet adhesion, platelet activation, thrombus structure and fibrin clot formation in real time using flowing whole
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Protocols

Laminin Coating Protocol for Cell Culture

Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. Protocols for coating coverslips to culture neurospheres and general cell culture are included.

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