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L9006

Sigma-Aldrich

Lectin from Ulex europaeus (gorse, furze)

FITC conjugate, lyophilized powder

Synonym(s):

Ulex europaeus agglutinin, UEA-I

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

conjugate

FITC conjugate

Quality Level

200

form

lyophilized powder

potency

<5 μg/mL agglutination activity

composition

Protein, ~10% Lowry

extent of labeling

2-5 mol FITC per mol protein

storage temp.

−20°C

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Biochem/physiol Actions

Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for L-fucose and UEA II has an affinity for N,N′-diacetylchitobiose. The mol. wt. of UEA I was initially found to be 170,000 daltons. Later reports indicate that UEA I may form aggregates at neutral and basic pH and that the correct mol. wt. is 68,000 daltons.

Packaging

Package size based on protein content.

Linkage

Conjugates are prepared from affinity purified lectin.

Physical form

Contains potassium phosphate buffer salts and NaCl

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Naoki Akanuma et al.
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We aimed to evaluate the contribution of acinar-to-ductal metaplasia (ADM) to the accumulation of cells with a ductal phenotype in cultured human exocrine pancreatic tissues and reveal the underlying mechanism. We sorted and cultured viable cell populations in human exocrine
Karla Lehle et al.
Artificial organs, 40(6), 577-585 (2015-10-30)
Multipotent progenitor cells were mobilized during pediatric extracorporeal membrane oxygenation (ECMO). We hypothesize that these cells also adhered onto polymethylpentene (PMP) fibers within the membrane oxygenator (MO) during adult ECMO support. Mononuclear cells were removed from the surface of explanted
Fenlong Xue et al.
Cell and tissue banking (2021-05-31)
The release of paracrine factors from endothelial progenitor cell (EPC) sheet is a central mechanism of tissue repair. The purpose of this study was to constuct the rat bone marrow derived-endothelial progenitor cell (BM-EPCs) sheet and investigate invest the role
Jing Ai et al.
Molecular medicine reports, 17(4), 5814-5820 (2018-02-28)
The viability of endothelial progenitor cells (EPCs) as a therapeutic treatment for neovascularization (NV) was subject to investigation in the present study. Furthermore, endostatin has previously been demonstrated to be an inhibitor of angiogenesis and a suppressant of vascular leakage.
Zoe E Clayton et al.
International journal of cardiology, 234, 81-89 (2017-02-18)
Endothelial cells derived from human induced pluripotent stem cells (iPSC-ECs) promote angiogenesis, and more recently induced endothelial cells (iECs) have been generated via fibroblast trans-differentiation. These cell types have potential as treatments for peripheral arterial disease (PAD). However, it is
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