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MAK196

Sigma-Aldrich

Malate Dehydrogenase Assay Kit

Sufficient for 100 Colorimetric tests

Synonym(s):

MDH Activity Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

detection method

colorimetric

relevant disease(s)

neurological disorders

storage temp.

−20°C

Gene Information

human ... MDH1(4190) , MDH1B(130752) , MDH2(4191)
mouse ... MDH1(17449) , MDH1B(76668) , MDH2(17448)
rat ... MDH1(24551) , MDH1B(316444) , MDH2(81829)

Related Categories

General description

Malate dehydrogenase (MDH; EC 1.1.1.37) is an NAD oxidoreductase that catalyzes the NAD/NADH-dependent interconversion of malate and oxalacetate. This reaction is critical to maintain the malate/aspartate shuttle across the mitochondrial membrane and Krebs tricarboxylic acid cycle in the mitochondrial matrix. Increased MDH activity has been observed in neurodegenerative diseases such as Alzheimer′s disease.

Application

Malate Dehydrogenase Assay Kit has been used in LDH (lactate dehydrogenase) and malate dehydrogenase (MDH1) assays. It has also been used to estimate isocitrate dehydrogenase and succinate dehydrogenase activities.

Suitability

Suitable for the detection of Malate Dehydrogenase activity in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.

Principle

The Malate Dehydrogenase Assay Kit provides a simple and sensitive procedure for measuring MDH activity in a variety of tissues, cell cultures, and isolated mitochondria. MDH activity is determined by generating a product with absorbance at 450 nm proportional to the enzymatic activity present. One unit of MDH is the amount of enzyme that generates 1.0 μmole of NADH per minute at 37 °C and pH 9.5.

Kit Components Only

Product No.
Description
  • MDH Assay Buffer
  • MDH Substrate
  • MDH Enzyme Mix
  • MDH Developer
  • NADH Standard
  • MDH Positive Control

replaced by

Product No.
Description
Pricing

Pictograms

Corrosion
GHS05

Signal Word

Danger

Hazard Statements

H314

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B

Storage Class Code

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

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Identification of a small molecule inhibitor of 3-phosphoglycerate dehydrogenase to target serine biosynthesis in cancers.
Mullarky E, et al.
Proceedings of the National Academy of Sciences of the USA, 113(7), 1778-1783 (2016)
Jinliang Wang et al.
Scientific reports, 9(1), 12300-12300 (2019-08-25)
The human mitochondrial heat shock protein 60 (hsp60) is a tetradecameric chaperonin that folds proteins in the mitochondrial matrix. An hsp60 D3G mutation leads to MitCHAP-60, an early onset neurodegenerative disease while hsp60 V72I has been linked to SPG13, a
Two plant-derived aporphinoid alkaloids exert their antifungal activity by disrupting mitochondrial iron-sulfur cluster biosynthesis.
Tripathi S K, et al.
The Journal of Biological Chemistry, jbc-M117 (2017)
Nan Nan et al.
Plant biotechnology journal, 18(1), 172-184 (2019-06-05)
Salinity is an important environmental factor that adversely impacts crop growth and productivity. Malate dehydrogenases (MDHs) catalyse the reversible interconversion of malate and oxaloacetate using NAD(H)/NADP(H) as a cofactor and regulate plant development and abiotic stress tolerance. Vitamin B6 functions
Anna Antolak et al.
Journal of cellular biochemistry, 118(12), 4323-4330 (2017-04-22)
Drug dependence is an escalating problem worldwide and many efforts are being made to understand the molecular basis of addiction. The morphine model is widely used in these investigations. To date, at least 29 studies exploring the influence of morphine
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Protocols

Enzymatic Assay of Malic Dehydrogenase

Spectrophotometric assay evaluates malic dehydrogenase activity using bovine heart enzyme with critical histidine residue at active site.

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