N6658

Nutrient Mixture F-12 Ham

With ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

• Synonym(s): Ham’s F-12
• MDL number: MFCD00217417
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• product name: Nutrient Mixture F-12 Ham, With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• components: L-glutamine: yes; HEPES: no; phenol red: yes; sodium pyruvate: 0.11 g/L; NaHCO₃: yes
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Ham′s Nutrient Mixtures were mainly created to support the clonal growth of numerous clones of Chinese hamster ovary (CHO) cells and clones of HeLa and mouse L-cells. It is also used to grow primary rat hepatocytes and rat prostate epithelial cells. Ham′s F-12 is used as the medium of choice for a clonal toxicity assay using CHO cells.

Application

Nutrient Mixture F-12 Ham has been used:

• to culture Chinese hamster ovary cells overexpressing insulin receptor (CHO-IR)
• to culture LAN-1 cells
• to maintain SUM149, SUM159, SUM190, FC-IBC02, BCX010, and IBC3 cells
• with Dulbecco’s modified Eagle’s medium (DMEM) media in a 1:1 ratio to culture oral cavity squamous cell carcinoma (OSCC) cell lines.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable