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Key Documents

P1598

Sigma-Aldrich

PCR 20 bp Low Ladder

for electrophoresis of PCR fragments

Synonym(s):

20 bp Low Ladder, Marker for DNA gel electrophoresis

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About This Item

UNSPSC Code:
41141827
NACRES:
NA.25

grade

for molecular biology

form

liquid

usage

80 uses

suitability

suitable for electrophoresis (DNA)

storage temp.

2-8°C

Related Categories

General description

Sigma′s PCR 20 bp Low Ladder contains 50 dsDNA fragments from 50 – 1,000 bases, evenly spaced in 20 bp repeats. Five μl of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.

Application

PCR 20 bp Low Ladder has been used as a marker for DNA gel electrophoresis.

Components

Sigma′s PCR 20 bp Low Marker is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Other Notes

Contains 50 bands, 20–1,000 bp in exact 20 bp repeats.
For optimal resolution, the recommended agarose gel concentration is 2.5%.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Primary mediastinal B-cell lymphoma: detection of BCL2 gene rearrangements by PCR analysis and FISH.
Dunphy CH, et al.
Journal of Hematopathology, 1, 77-84 (2008)

Articles

Gel Electrophoresis Markers

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

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