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RAB0257

Sigma-Aldrich

Mouse IL-13 ELISA Kit

for serum, plasma and cell culture supernatant

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

mouse

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type serum
sample type cell culture supernatant(s)
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 6 pg/mL
standard curve range: 8023-2000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Il13(16163)

General description

The Mouse IL-13 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-13 in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Mouse IL-13

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Mouse IL-13 ELISA Kit has been used to measure the concentration of interleukin (IL)-13 in serological sampleand bronchoalveolar lavage fluids (BALFs).

Biochem/physiol Actions

Interleukin 13 (IL13) is up-regulated during parasitic infections and participates in host′s protective immunity against Schistosoma japonicum. This protein promotes eosinophil activation resulting in elevated specific IgE against adult and larval antigens. During acute infection it promotes egg expulsion through increased intestinal contractility. It acts as a liver protective agent where it promotes the formation of liver granulomas in a CD4+ T cell-mediated manner, which results in liver fibrosis. In airway disorders, such as asthma, chronic obstructive pulmonary disease (COPD), and cystic fibrosis lung disease, IL13 promotes the formation of airway goblet cells, resulting in excess mucus secretion, which in turn, is regulated by IL13-activated autophagy.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS
  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Paeoniflorin targets apoptosis and ameliorates fibrosis in murine schistosomiasis mansoni: A novel insight
El-Aal N, et al.
Experimental Parasitology, 183, 23-32 (2017)
Therapeutic effects of liver soothing pingchuan formula decoction on experimental asthma in BALB/c mice via regulation of nerve growth factor-tyrosine kinase A pathway
Zhang XG, et al.
Molecular Medicine Reports, 17(5), 6977-6984 (2018)
Xin-Guang Zhang et al.
Molecular medicine reports, 17(5), 6977-6984 (2018-03-24)
The present study was designed to investigate the effects of liver soothing pingchuan formula decoction (LSPF) on experimental asthma in BALB/c mice and explore its potential molecular mechanisms. An animal model of asthma was established in BALB/c mice through sensitization and activation with
Arvind Chopra et al.
Arthritis & rheumatology (Hoboken, N.J.), 66(2), 319-326 (2014-02-08)
To evaluate whether chloroquine (CQ) is more effective than meloxicam for treating early musculoskeletal pain and arthritis following acute chikungunya (CHIK) virus infection. During the 2006 CHIK epidemic, 509 rural community cases of acute CHIK virus infection were identified in
IL13 activates autophagy to regulate secretion in airway epithelial cells.
Dickinson JD et al
Autophagy, 12(2), 397-409 (2016)
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