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S5394

Sigma-Aldrich

Lipoprotein Deficient Serum from fetal calf

frozen liquid

Synonym(s):

Fetal Calf Serum, Lipoprotein Deficient Serum

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.61

biological source

bovine fetus

Quality Level

200

form

frozen liquid

concentration

≥14 mg protein/mL Biuret

technique(s)

cell culture | mammalian: suitable
cell culture | stem cell: suitable

impurities

<1 ng/mg protein endotoxin
<5 % of normal lipoprotein

shipped in

dry ice

storage temp.

−20°C

General description

Studies show that the production of sterols is reduced in human fibroblasts cultured in the presence of lipoprotein deficient serum.

Application

Lipoprotein Deficient Serum from fetal calf has been used as a supplement in culture media to grow Chinese hamster ovary (CHO)-derived cells, thymic lobes and human peripheral blood mononuclear cells (PBMCs).

Preparation Note

Aseptically filtered, 0.2 μm
Dialyzed against Dulbecco′s phosphate buffered saline, pH 7.4-7.5

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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P M Giles et al.
Clinica chimica acta; international journal of clinical chemistry, 113(2), 183-191 (1981-06-18)
The biosynthesis and efflux of sterols from cells into the medium were investigated in skin fibroblasts from a control, a patient with obligate heterozygous familial hypercholesterolaemia and a patient with the homozygous condition. The behaviour of the cells was studied
Brian T Emmer et al.
PLoS genetics, 17(1), e1009285-e1009285 (2021-01-30)
Hypercholesterolemia is a causal and modifiable risk factor for atherosclerotic cardiovascular disease. A critical pathway regulating cholesterol homeostasis involves the receptor-mediated endocytosis of low-density lipoproteins into hepatocytes, mediated by the LDL receptor. We applied genome-scale CRISPR screening to query the
Ning Huangfu et al.
Frontiers in cell and developmental biology, 9, 687169-687169 (2021-07-23)
Atherosclerosis (AS), characterized by cholesterol overloaded-macrophages accumulation and plaque formation in blood vessels, is the major cause of cardiovascular disease. Transactive response DNA-binding protein∼43 kDa (TDP43) has recently been identified as an independent driver of neurodegenerative diseases through triggering inflammatory
Liliana M R Silva et al.
Frontiers in cellular and infection microbiology, 12, 809606-809606 (2022-03-01)
Apicomplexan parasites are considered as defective in cholesterol synthesis. Consequently, they need to scavenge cholesterol from the host cell by either enhancing the uptake of extracellular cholesterol sources or by upregulating host cellular de-novo biosynthesis. Given that Eimeria bovis macromeront
Ying Wang et al.
Cell death discovery, 7(1), 201-201 (2021-08-06)
Coenzyme Q (CoQ) is a lipid-like mobile electron transporter of the mitochondrial respiratory chain. Patients with partial loss-of-function mutations in the CoQ biosynthesis pathway suffer from partial primary CoQ deficiency (MIM 607426). This leads to mitochondrial dysfunction, which presents like
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