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Key Documents

S6657

Sigma-Aldrich

Superdex®

Superdex®75 Prep Grade

Synonym(s):

Superdex Resin

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About This Item

MDL number:
UNSPSC Code:
23151817
NACRES:
NA.56

form

suspension (20% aqueous ethanol)

technique(s)

protein array: suitable

matrix

cross-linked agarose

particle size

24-44 μm

pore size

3-70 kDa MW range (proteins)
500-30,000 Da MW range (dextrans)

operating pH range

1-14(short term)
3-12(long term)

storage temp.

2-8°C

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General description

Combines the high selectivity of a Sephadex matrix with the chemical and physical stability of crosslinked agarose.

Application

Superdex® is used for protein chromatography, gel filtration chromatography, gel filtration media, resins and separation media. Superdex® has been used for the purification and characterization of a novel laccase from the edible mushroom Hericium coralloides. Superdex® has also been used for the purification and mechanism exploration of a potential human hepatocellular carcinoma inhibitor from Bauhinia purpurea L. seeds.
Optimized for high resolution preparative separations by size exclusion chromatography. Recommended fractionation ranges are listed for globular proteins and dextrans.

Legal Information

Superdex is a registered trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

104.0 °F

Flash Point(C)

40 °C


Certificates of Analysis (COA)

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Toshiro Matsunaga et al.
Plant physiology, 134(1), 339-351 (2003-12-13)
Borate ester cross-linking of the cell wall pectic polysaccharide rhamnogalacturonan II (RG-II) is required for the growth and development of angiosperms and gymnosperms. Here, we report that the amounts of borate cross-linked RG-II present in the sporophyte primary walls of
Cornelia Steinhauer et al.
Proteomics, 6(15), 4227-4234 (2006-07-11)
Antibody-based microarray is a novel technology with great promise in biomedicine that will provide unique means to perform global proteome analysis. In the process of designing the high-density antibody microarrays required, several critical key issues have been identified that remain
M Belew et al.
Journal of chromatography. A, 679(1), 67-83 (1994-09-09)
Recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF), produced as inclusion bodies in genetically transformed Escherichia coli cells was purified to homogeneity by a three-step chromatographic procedure involving hydrophobic interaction, ion exchange and gel filtration. Each purification step is reproducible and well
M Maiorino et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 12(13), 1359-1370 (1998-10-08)
Selenium deficiency is known to be associated with male infertility, and the selenoprotein PHGPx has been shown to increase in rat testis after puberty and to depend on gonadotropin stimulation in hypophysectomized rats [Roveri et al. (1992) J. Biol. Chem.
Xindu Geng et al.
Journal of biotechnology, 113(1-3), 137-149 (2004-09-24)
A new technology for renaturation with simultaneous purification of the recombinant human interferon-gamma (rhIFN-gamma) in downstream of biotechnology is presented. The strategies to develop the new technology in industry scale were suggested. Based on chemical equilibrium and molecular interactions, the

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