Skip to Content
Merck
All Photos(3)

Key Documents

SRP0300

Sigma-Aldrich

TGF-β1 Latent human

recombinant, expressed in FreeStyle™ 293-F cells, ≥98% (SDS-PAGE)

Synonym(s):

LAP, TGFb1, TGFbeta1, latency-associated peptide, transforming growth factor beta-1

Sign Into View Organizational & Contract Pricing
All Photos(3)

About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

human

recombinant

expressed in FreeStyle™ 293-F cells

Assay

≥98% (SDS-PAGE)

form

aqueous solution

mol wt

25 kDa

packaging

pkg of 5 μg

manufacturer/tradename

Invitrogen

storage condition

avoid repeated freeze/thaw cycles

NCBI accession no.

NM_000660

UniProt accession no.

Q14766

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... LTBP1(4052)

General description

Human TGFβ1 (GenBank Accession No. NM_000660), amino acids 30-390 (end) with Cys33Ser mutation and N-terminal His-tag, disulfide-linked homodimers of TGFβ1 LAP and mature TGFβ1, MW= ~75kDa and 25kDa, under non-reducing conditions.
LTBP1 (latent transforming growth factor β binding protein 1) belongs to the LTBP family of proteins. The protein has 18 epidermal growth factor (EGF)-like repeats and four LTBP-like repeats. The gene is mapped to human chromosome 2p22.3.

Application

Useful for cell culture and for the study of signaling and apoptotic pathways.

Biochem/physiol Actions

LTBP1 (latent transforming growth factor β binding protein 1) is an extracellular matrix protein which also controls TGF-β (transforming growth factor β) bioavailability. LTBP1 is mainly responsible for the folding and secretion of TGF-β, thereby adding it to the extracellular matrix. It is a target of HTRA1 (high temperature requirement protein A1), a serine protease involved in cellular signaling.

Physical form

Lyophilized from a 0.2 μM filtered, aqueous solution.

Reconstitution

Reconstitute in sterile PBS with 0.1% BSA. Stability: >6 months at –70°C (as a solid). Store reconstituted protein in aliquots at –80°C for up to 3 months. Avoid freeze/thaw cycles.

Legal Information

FreeStyle is a trademark of Invitrogen Corp.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

NMR spectroscopic and bioinformatic analyses of the LTBP1 C-terminus reveal a highly dynamic domain organisation.
Robertson IB, et al.
PLoS ONE, 9, e87125-e87125 (2014)
Cerebral small vessel disease-related protease HtrA1 processes latent TGF-? binding protein 1 and facilitates TGF-? signaling.
Beaufort N, et al.
Proceedings of the National Academy of Sciences of the USA, 111, 16496-16501 (2014)
A suite of novel human spermatozoal RNAs.
Ostermeier GC, et al.
Journal of Andrology, 26, 70-74 (2005)
Association of the small latent transforming growth factor-beta with an eight cysteine repeat of its binding protein LTBP-1.
Saharinen J, et al.
The Embo Journal, 15, 245-253 (1996)
L Bourdrel et al.
Protein expression and purification, 4(2), 130-140 (1993-04-01)
A partial cDNA clone encoding the gene for human transforming growth factor-beta 1 (TGF-beta 1) was isolated from a human bladder carcinoma cell line (5637) cDNA library. Following restriction enzyme processing and ligation of synthetic oligonucleotide linkers, the gene was
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service