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G004494

HPLC Analysis of Cyclophosphamide Enantiomers on Astec® CHIROBIOTIC® T

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)
mobile phase: [A] water; [B] ethanol; [C] THF; (90:5:5, A:B:C)
flow rate: 0.75 mL/min
column temp.: 23 °C
detector: UV, 205 nm
injection: 5 μL
sample: 1 mg/mL in methanol

suitability

application for HPLC

application(s)

pharmaceutical (small molecule)

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

Analyte

Description

    Cyclophosphamide monohydrate, bulk package

standard

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Description
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