G005698
HPLC Analysis of Fat Soluble Vitamins on Ascentis® RP-Amide
application for HPLC
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About This Item
technique(s)
HPLC: suitable
test parameters
column: Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 μm particles (565324-U)
mobile phase: acetonitrile
flow rate: 2.0 mL/min
column temp.: 35 °C
detector: UV, 215 nm
injection: 20 μL
suitability
application for HPLC
application(s)
vitamins, nutraceuticals, and natural products
Analysis Note
The Ascentis RP-Amide is shown to be suitable for the analysis of fat soluble vitamins, alpha tocopherol (vitamin E) and cholecalciferol (vitamin D3 ).
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Analyte
Description
Vitamin D3 solution, 1 mg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®
(±)-α-Tocopherol, analytical standard
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