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D9143

Sigma-Aldrich

o-Dianisidine

peroxidase substrate, chromogenic, powder

Synonym(s):

3,3′-Dimethoxybenzidine, Fast Blue B

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About This Item

Empirical Formula (Hill Notation):
C14H16N2O2
CAS Number:
119-90-4
Molecular Weight:
244.29
Beilstein:
1879884
EC Number:
204-355-4
MDL number:
MFCD00008372
UNSPSC Code:
12352204
PubChem Substance ID:
24894258
NACRES:
NA.83

Product Name

o-Dianisidine, peroxidase substrate

Quality Level

200

form

powder

solubility

H2O: slightly soluble
aqueous acid: soluble

SMILES string

COc1cc(ccc1N)-c2ccc(N)c(OC)c2

InChI

1S/C14H16N2O2/c1-17-13-7-9(3-5-11(13)15)10-4-6-12(16)14(8-10)18-2/h3-8H,15-16H2,1-2H3

InChI key

JRBJSXQPQWSCCF-UHFFFAOYSA-N

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Application

o-Dianisidine has been used for neutrophil myeloperoxidase activity (MPO) assay. It has also been used for staining erythrocytes.
as a constituent in neutral benzidine staining of dried blood smears

Caution

Darkens in storage.

Warning

Probably carcinogenic.

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H302,H350

Hazard Classifications

Acute Tox. 4 Oral - Carc. 1B

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

402.8 °F - closed cup

Flash Point(C)

206 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

CAS No.

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Certificates of Analysis (COA)

Lot/Batch Number
SHBP1768
SHBP6653
MKCC7501
MKBX3619V
MKBS1689V

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FIP200 is required for the cell-autonomous maintenance of fetal hematopoietic stem cells
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Demy DL, et al.
Journal of Cell Science, jcs-203471 (2017)
Amnion epithelial cells promote lung repair via lipoxin A4
Tan JL, et al.
Stem Cells Translational Medicine, 6(4), 1085-1095 (2017)
RNAi-mediated silencing of TNF-alphas converting enzyme to down-regulate soluble TNF-alpha production for treatment of acute and chronic colitis
Song Y, et al.
Journal of Controlled Release : Official Journal of the Controlled Release Society, 239, 231-241 (2016)
D M Lehmann et al.
Access microbiology, 1(10), e000065-e000065 (2020-09-26)
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NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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