R7757
Red Blood Cell Lysing Buffer Hybri-Max™
Liquid, sterile-filtered, suitable for hybridoma
Synonym(s):
RBC Lysing Buffer
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.75
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Product Name
Red Blood Cell Lysing Buffer Hybri-Max™, liquid, sterile-filtered, suitable for hybridoma
sterility
sterile-filtered
Quality Level
form
liquid
technique(s)
cell culture | hybridoma: suitable
impurities
endotoxin, tested
Application
Recommended for use by adding 1 mL of buffer to a cell pellet (cell pellet = 1 spleen or 100-200 million cells). Gently mix for 1 minute. Dilute the buffer with 15-20 mL of medium or salt solution. Centrifuge at 250-500 × g for 7 minutes and decant the supernatant. Cells may be diluted and prepared for counting or fusion. If lysis is incomplete, steps 1-4 may be repeated.
Biochem/physiol Actions
Red Blood Cell Lysing Buffer has been developed for use in hybridoma protocols to remove red blood cells from mouse splenocyte suspensions before fusion. It is also useful in systems where it may be desirable to remove red blood cells from cell suspensions, such as whole blood.
Components
Contains 8.3 g/L ammonium chloride in 0.01 M Tris-HCl buffer.
Other Notes
Note: This product is intended for the removal of red blood cells from mice. This product may not be appropriate for the lysis of red blood cells of other animals. The suitability of the product in any application other than mouse splenocytes must be determined by the researcher.
Legal Information
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
EU REACH Annex XVII (Restriction List)
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