SHRNA
MISSION® shRNA Product Offerings
Order Custom and Predesigned shRNA
Synonym(s):
RNAi, Custom shRNA, High-throughput shRNA, MISSION® shRNA, Short hairpin RNA, Small hairpin RNA, inducible shRNA, shRNA, shRNA arrayed, shRNA gene sets, shRNA library, shRNA panels, shRNA pools, targeted integration shRNA
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MISSION® shRNA Products
Individual MISSION® shRNA Clones
- Our online tool allows you to view clone-specific validation information and build your own clone sets.
- The individual clones are available in three formats: bacterial glycerol stock, purified plasmid DNA and lentiviral transduction particles
- Locate shRNA clones for your specific gene of interest by clicking 'Search'
- Choose from over 315,000 pre-cloned shRNA constructs for both human and mouse.
- Learn More
Bacteria
DNA
Lentivirus
MISSION® shRNA Gene Family Sets
Choose from 25 Pre-Designed shRNA Gene Panels
- All TRC shRNA clones have been methodically mapped to each current gene set by our Bioinformatics team.
- Gene family sets for human (22) and mice (3) include popular kinases, phosphatases, GPCRs, tyrosine kinases, and epigenetic regulators.
- Any panel can be arrayed in either 96- or 384- well plate formats
- All gene family sets can be used at the benchtop for focused target discovery and pathway analysis.
Bacteria
DNA
Lentivirus
Create your own panel.
Different gene pathway of interest? Want a more complete list?
Submit a request.
- Gene family sets are fully customizable allowing for complete flexibility to add or delete clone or gene content.
MISSION® shRNA Libraries
Arrayed genome-wide shRNA libraries
- Most extensive coverage: Pre-cloned genome-wide arrayed libraries contain 245,000+ clone shRNA library targeting 20,000+ human and 21,000+ mouse genes.
- High-throughput manufacturing: World class high-throughput viral manufacturing.
- Flexible format options: Entire libraries or single gene sets offered in glycerol stock, DNA, or viral format.
- Extended Coverage=Average of 5 - 6 clones targeting each gene.
Bacteria
DNA
Lentivirus
Pooled shRNA Libraries
Whole genome shRNA screening for a fraction of the cost.
- Affordable - Bench top discovery without robotics or liquid handling.
- Comprehensive - Trusted TRC (The RNAi Consortium) content with shRNA taregting 20,000+ human or 21,000+ mouse genes including TRC 1.5 and 2.0 (unique and validated) add-ons which are exclusive to Sigma.
- Universal - Simplified whole genome RNAi screening in standard or difficult to expand cell lines.
- Versatile - Compatible with FACS and most downstream fluorescent assays.
Lentivirus
Custom MISSION® shRNA
Fully customizable shRNA sequence, vector, and lentiviral manufacturing
- Select by methods other than puromycin, including neomycin (G418) and FACS via reporters.
- Confirm your lentiviral delivery through a fluorescent reporter.
- Customize your shRNA sequence.
- Eliminate tedious cloning and viral production.
- Service includes: shRNA design, cloning, sequence verifications, DNA quantitation, and titer determination.
Custom Capabilities: Bringing you more flexibility
- Contract manufacturing: Send us your clones for lentiviral production! We accommodates high-throughput projects, private collections, and alternate libraries. Please request a quote or contact if interested.
DNA
Lentivirus
Create Your Own shRNA Pool
Focus on the genes essential to your research by creating your own custom shRNA pool
- Create your custom shRNA pool for maximum return of relevant hits
- Customize your volume and aliquoting needs to further enhance your ability to rapidly screen multiple cell lines.
- Titers and volumes adequate for in vitro, in vivo and xenograft applications.
- Partner with Sigma's next-generation sequencing services for data deconvolution and maximum data return from your screening efforts.
Lentivirus
Services
Deconvolution of shRNA Pools
- Sigma's deconvolution service lets you easily identify the genes that impact your pooled shRNA screen.
- Next-generation sequencing of clones gives a precise number of individual clone occurrence within a pooled shRNA sample.
- Comprehensive, reproducible results from pooled shRNA screens.
- Statistically robust and information-rich data.
- Learn More
shRNA Controls
Measure transduction and knockdown efficiency, or monitor off-target effects.
- Positive Transduction Controls: Empty Vector, TurboGFP, TagCFP, TagYFP, TagRFP, and TagFP365 Controls
- Positive Knockdown Controls: TurboGFP, eGFP, luciferase, B2M, and ARHGDIA controls
- Negative Controls: Empty Vector, Non-target, Turbo GFP, eGFP, and luciferase controls
- Learn More
DNA
Lentivirus
Delivery
Produce high-titer lentivirus using the MISSION® Lentiviral Packaging Mix
- Accelerate viral production by skipping tedious but critical multivariable plasmid optimization.
- Produce high titer lentivirus with MISSION® shRNA plasmids with the included easy to use protocol.
- Safely produce lentivirus utilizing the three plasmid system with self inactivating, long terminal repeat (SIN/LTR) regions.
- Choose between small or large scale lentivirus production with our conveniently sized 250 µl or 1.7 ml sizes.
Packaging Mix
Inducible MISSION® shRNA
Tight regulation meets great gene silencing
- IPTG-inducible shRNA pLKO-vectors allow for fast response and temporally-controlled gene silencing useful for essential or lethal gene knockdown.
- Proven to work in vivo and in vitro
- Single vector systems in 1X LacO and 3X LacO formats for effective inducible knockdown
- Available on demand service includes: shRNA design(choose any TRC clone or custom sequence), cloning, sequence verification, DNA quantitation, and titer determination.
DNA
Lentivirus
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This screening guide covers how to choose a cell line, a screening library, and experimental conditions as well as tips for designing and performing your experiment.
RNAi Consortium (TRC): Collaborative effort among academic labs and biotech/pharma institutes advancing RNA interference research.
RNAi Consortium (TRC): Collaborative effort among academic labs and biotech/pharma institutes advancing RNA interference research.
RNAi Consortium (TRC): Collaborative effort among academic labs and biotech/pharma institutes advancing RNA interference research.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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